TDRD5 Polyclonal antibody
TDRD5 Polyclonal Antibody for WB, IP, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
WB, IP, IHC, ELISA
Conjugate
Unconjugated
Cat no : 25239-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | mouse testis tissue |
Positive IP detected in | mouse testis tissue |
Positive IHC detected in | mouse testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:200-1:1000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
25239-1-AP targets TDRD5 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse samples.
Tested Reactivity | human, mouse |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | TDRD5 fusion protein Ag18896 相同性解析による交差性が予測される生物種 |
Full Name | tudor domain containing 5 |
Calculated molecular weight | 981 aa, 110 kDa |
Observed molecular weight | ~116 kDa |
GenBank accession number | BC130532 |
Gene symbol | TDRD5 |
Gene ID (NCBI) | 163589 |
RRID | AB_2879981 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
TDRD5, also named as Tudor domain-containing protein 5, is a 981 amino acid protein, which contains one Tudor domain and three HTH OST-type domains. TDRD5 localizes in the cytiplasm and belongs to the TDRD5 family. TDRD5 is required during spermiogenesis to participate in the repression transposable elements and prevent their mobilization, which is essential for the germline integrity.
Protocols
Product Specific Protocols | |
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WB protocol for TDRD5 antibody 25239-1-AP | Download protocol |
IHC protocol for TDRD5 antibody 25239-1-AP | Download protocol |
IP protocol for TDRD5 antibody 25239-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |