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- KD/KO Validated
TDRKH specific Polyclonal antibody
TDRKH specific Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (1)
Applications
WB, IHC, IF/ICC, IP, RIP, ELISA
Conjugate
Unconjugated
Cat no : 13528-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | NCCIT cell, mouse brain tissue |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | human gliomas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 12 publications below |
| IHC | See 3 publications below |
| IF | See 11 publications below |
| IP | See 4 publications below |
| RIP | See 1 publications below |
Product Information
13528-1-AP targets TDRKH specific in WB, IHC, IF/ICC, IP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, bovine |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | TDRKH specific fusion protein Ag4422 相同性解析による交差性が予測される生物種 |
| Full Name | tudor and KH domain containing |
| Calculated molecular weight | 606 aa, 67 kDa |
| Observed molecular weight | 67 kDa |
| GenBank accession number | BC032690 |
| Gene symbol | TDRKH |
| Gene ID (NCBI) | 11022 |
| RRID | AB_2303299 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Tudor and KH domain-containing protein(TDRKH)Tudor domains are protein modules that mediate protein-protein interactions, potentially by binding to methylated ligands. Both KH and tudor domains are involved in binding RNA or single-strand DNA. Tudor also is a germ cell-specific protein with multiple Tudor domains and is involved in germ plasm formation and germ cell specification. TDRKH is one Tudor protein with a single Tudor domain.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for TDRKH specific antibody 13528-1-AP | Download protocol |
| IHC protocol for TDRKH specific antibody 13528-1-AP | Download protocol |
| IF protocol for TDRKH specific antibody 13528-1-AP | Download protocol |
| IP protocol for TDRKH specific antibody 13528-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Cell Biol Ribosomes guide pachytene piRNA formation on long intergenic piRNA precursors. | ||
Sci Adv Global ubiquitylation analysis of mitochondria in primary neurons identifies endogenous Parkin targets following activation of PINK1. | ||
Nat Commun PNLDC1 is essential for piRNA 3' end trimming and transposon silencing during spermatogenesis in mice.
| ||
EMBO J Tdrkh is essential for spermatogenesis and participates in primary piRNA biogenesis in the germline.
| ||
Proc Natl Acad Sci U S A Mouse Piwi interactome identifies binding mechanism of Tdrkh Tudor domain to arginine methylated Miwi. |
