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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TEAD3 Monoclonal antibody (68464-1-Ig)

    WB analysis using 68464-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68464-1-Ig (TEAD3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using TEAD3 Monoclonal antibody (68464-1-Ig)

    WB analysis using 68464-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68464-1-Ig (TEAD3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

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TEAD3 Monoclonal antibody

TEAD3 Monoclonal Antibody for WB, ELISA
Cat No. 68464-1-Ig
Clone No.2E6A12

Host / Isotype

Mouse / IgG1

Reactivity

Human, pig

Applications

WB, ELISA

Synonyms

DTEF 1, ETFR 1, TEA domain family member 3, TEAD 3, TEAD3, TEAD5, TEF 5, TEF5

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHCT 116 cells, JAR cells, HepG2 cells, U2OS cells, pig heart tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68464-1-Ig targets TEAD3 in WB, ELISA applications and shows reactivity with Human, pig samples.

Tested Reactivity Human, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen TEAD3 fusion protein Ag4407 相同性解析による交差性が予測される生物種
Full Name TEA domain family member 3
Calculated molecular weight 435 aa, 49 kDa
Observed molecular weight50 kDa
GenBank accession numberBC027877
Gene Symbol TEAD3
Gene ID (NCBI) 7005
RRIDAB_3085175
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ99594
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TEAD3, others named transcription enhancer factor 5(TEF5), is a member of the family of transcription factors that plays a key role in the Hippo signaling pathway, suggesting involvement in organ and tumor suppression by restricting proliferation and promoting. TEAD3 contains the TEA/ATTS DNA-binding domain. Human TEAD3 has a strong mRNA expression in the placenta and choriocarcinoma cells(JEG-3) but is low in Hela, HepG2, and MCF-7.

Protocols

Product Specific Protocols
WB protocol for TEAD3 antibody 68464-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols