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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

TEC Polyclonal antibody

TEC Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 18085-1-AP

Synonyms

PSCTK4, TEC, tec protein tyrosine kinase, Tyrosine protein kinase Tec

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TEC Polyclonal antibody (18085-1-AP)

    WB analysis using 18085-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 18085-1-AP (TEC antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using TEC Polyclonal antibody (18085-1-AP)

    WB analysis using 18085-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 18085-1-AP (TEC antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of K-562 cells using TEC Polyclonal antibody (18085-1-AP)

    WB analysis of K-562 using 18085-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 18085-1-AP (TEC antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using TEC Polyclonal antibody (18085-1-AP)

    IHC staining of human lung cancer using 18085-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 18085-1-AP (TEC antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inK-562 cells, mouse kidney tissue, Jurkat cells, rat kidney tissue
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 18085-1-AP is TEC Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TEC fusion protein Ag12804 相同性解析による交差性が予測される生物種
Full Name tec protein tyrosine kinase
Calculated molecular weight 631 aa, 74 kDa
Observed molecular weight 74 kDa
GenBank accession numberBC101711
Gene symbol TEC
Gene ID (NCBI) 7006
RRIDAB_2878494
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for TEC antibody 18085-1-APDownload protocol
IHC protocol for TEC antibody 18085-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols