Validation Data Gallery

Filter: AllWBIHCIF/ICC

WB analysis of human brain using 18968-1-AP
human brain tissue were subjected to SDS PAGE followed by western blot with 18968-1-AP (TEKT1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of human testis using 18968-1-AP
human testis tissue were subjected to SDS PAGE followed by western blot with 18968-1-AP (TEKT1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of rat testis using 18968-1-AP
rat testis tissue were subjected to SDS PAGE followed by western blot with 18968-1-AP (TEKT1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
IHC staining of human testis using 18968-1-AP
Immunohistochemical analysis of paraffin-embedded human testis using 18968-1-AP (TEKT1 antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human testis using 18968-1-AP
Immunohistochemical analysis of paraffin-embedded human testis using 18968-1-AP (TEKT1 antibody) at dilution of 1:100 (under 40x lens).
IF Staining of HepG2 using 18968-1-AP
Immunofluorescent analysis of HepG2 cells, using TEKT1 antibody 18968-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
IF Staining of MCF-7 using 18968-1-AP
Immunofluorescent analysis of MCF-7 cells, using TEKT1 antibody 18968-1-AP at 1:25 dilution and FITC- labeled FITC-Donkey anti-Rabbit IgG (green).

Featured Product
KD/KO Validated

TEKT1 Polyclonal antibody

TEKT1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 18968-1-AP

Publications(4)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

Tektin-1

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Overview

Tested Applications
Product Information
Publications (4)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	human brain tissue, human testis tissue, rat testis tissue
Positive IHC detected in	human testis tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells, MCF-7 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:3000
Immunohistochemistry (IHC)	IHC : 1:20-1:200
Immunofluorescence (IF)/ICC	IF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 3 publications below
IF	See 2 publications below
IP	See 1 publications below

Product Information

18968-1-AP targets TEKT1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	TEKT1 fusion protein Ag5173 相同性解析による交差性が予測される生物種
Full Name	tektin 1
Calculated molecular weight	48 kDa
Observed molecular weight	48-50 kDa
GenBank accession number	BC014599
Gene Symbol	TEKT1
Gene ID (NCBI)	83659
RRID	AB_2203324
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q969V4
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for TEKT1 antibody 18968-1-AP	Download protocol
IHC protocol for TEKT1 antibody 18968-1-AP	Download protocol
IF protocol for TEKT1 antibody 18968-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB	Curr Biol CCDC176 stabilizes microtubule doublets 1 and 9 to ensure proper sperm movement Authors - Chao Liu View Article
mouse	WB,IP	Cell Mol Life Sci Tektin bundle interacting protein, TEKTIP1, functions to stabilize the tektin bundle and axoneme in mouse sperm flagella Authors - Xin-Yan Geng View Article
human	WB,IF	Hum Mol Genet Functional characterization of tektin-1 in motile cilia and evidence for TEKT1 as a new candidate gene for motile ciliopathies. Authors - Rebecca Ryan KD Validated View Article
mouse	IF	Dev Dyn Proteomic profiling of TBC1 domain family member 21-null sperms reveals the critical roles of TEKT 1 in their tail defects Authors - Pei-Yi Pan View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of human brain using 18968-1-AP

WB analysis of human testis using 18968-1-AP

WB analysis of rat testis using 18968-1-AP

IHC staining of human testis using 18968-1-AP

IHC staining of human testis using 18968-1-AP

IF Staining of HepG2 using 18968-1-AP

IF Staining of MCF-7 using 18968-1-AP

TEKT1 Polyclonal antibody

TEKT1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

CCDC176 stabilizes microtubule doublets 1 and 9 to ensure proper sperm movement

Tektin bundle interacting protein, TEKTIP1, functions to stabilize the tektin bundle and axoneme in mouse sperm flagella

Functional characterization of tektin-1 in motile cilia and evidence for TEKT1 as a new candidate gene for motile ciliopathies.

Proteomic profiling of TBC1 domain family member 21-null sperms reveals the critical roles of TEKT 1 in their tail defects