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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

TEKT4 Polyclonal antibody

TEKT4 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 17058-1-AP

Synonyms

TEKT4, tektin 4

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of MDA-MB-453s cells using TEKT4 Polyclonal antibody (17058-1-AP)

    WB analysis of MDA-MB-453s using 17058-1-AP

    MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with 17058-1-AP (TEKT4 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human testis tissue using TEKT4 Polyclonal antibody (17058-1-AP)

    WB analysis of human testis using 17058-1-AP

    human testis tissue were subjected to SDS PAGE followed by western blot with 17058-1-AP (TEKT4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using TEKT4 Polyclonal antibody (17058-1-AP)

    IP experiment of HeLa using 17058-1-AP

    IP result of anti-TEKT4 (IP:17058-1-AP, 4ug; Detection:17058-1-AP 1:500) with HeLa cells lysate 1200ug.

  • Immunohistochemistry (IHC) staining of human kidney tissue using TEKT4 Polyclonal antibody (17058-1-AP)

    IHC staining of human kidney using 17058-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 17058-1-AP (TEKT4 antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human skin tissue using TEKT4 Polyclonal antibody (17058-1-AP)

    IHC staining of human skin using 17058-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin using 17058-1-AP (TEKT4 antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human lung tissue using TEKT4 Polyclonal antibody (17058-1-AP)

    IHC staining of human lung using 17058-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung using 17058-1-AP (TEKT4 antibody) at dilution of 1:100 (under 40x lens).

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Tested Applications

Positive WB detected inMDA-MB-453s cells, human testis tissue
Positive IP detected inHeLa cells
Positive IHC detected inhuman kidney tissue, human lung tissue, human skin tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17058-1-AP targets TEKT4 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TEKT4 fusion protein Ag10772 相同性解析による交差性が予測される生物種
Full Name tektin 4
Calculated molecular weight 435 aa, 51 kDa
Observed molecular weight 52 kDa
GenBank accession numberBC021716
Gene symbol TEKT4
Gene ID (NCBI) 150483
RRIDAB_2203334
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for TEKT4 antibody 17058-1-APDownload protocol
IHC protocol for TEKT4 antibody 17058-1-APDownload protocol
IP protocol for TEKT4 antibody 17058-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC,IF,CoIP

Nat Commun

Enriched variations in TEKT4 and breast cancer resistance to paclitaxel.

Authors - Yi-Zhou Jiang
View Article
mouseWB,IP

Cell Mol Life Sci

Tektin bundle interacting protein, TEKTIP1, functions to stabilize the tektin bundle and axoneme in mouse sperm flagella

Authors - Xin-Yan Geng
View Article
mouseIF

Dev Dyn

Proteomic profiling of TBC1 domain family member 21-null sperms reveals the critical roles of TEKT 1 in their tail defects

Authors - Pei-Yi Pan
View Article