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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

TEM1 Monoclonal antibody, PBS Only (Capture)

TEM1 Monoclonal Antibody for WB, IHC, Cytometric bead array, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

WB, IHC, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1F9B4

Cat no : 60170-1-PBS

Synonyms

CD248, TEM-1, Endosialin, CD248 molecule, endosialin, CD164L1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TEM1 Monoclonal antibody (60170-1-Ig)

    WB analysis using 60170-1-Ig (same clone as 60170-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 60170-1-Ig (TEM1 antibody) at dilution of 1:23000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60170-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HUVEC cells using TEM1 Monoclonal antibody (60170-1-Ig)

    WB analysis of HUVEC using 60170-1-Ig (same clone as 60170-1-PBS)

    HUVEC cells were subjected to SDS PAGE followed by western blot with 60170-1-Ig (TEM1 antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60170-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of COLO 320 cells using TEM1 Monoclonal antibody (60170-1-Ig)

    WB analysis of COLO 320 using 60170-1-Ig (same clone as 60170-1-PBS)

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 60170-1-Ig (TEM1 antibody at dilution of 1:1800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60170-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using TEM1 Monoclonal antibody (60170-1-Ig)

    IHC staining of human breast cancer using 60170-1-Ig (same clone as 60170-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60170-1-Ig (TEM1 antibody at dilution of 1:250 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60170-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using TEM1 Monoclonal antibody (60170-1-Ig)

    IHC staining of human breast cancer using 60170-1-Ig (same clone as 60170-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60170-1-Ig (TEM1 antibody at dilution of 1:250 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60170-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human renal cell carcinoma tissue using TEM1 Monoclonal antibody (60170-1-Ig)

    IHC staining of human renal cell carcinoma using 60170-1-Ig (same clone as 60170-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 60170-1-Ig (TEM1 antibody at dilution of 1:250 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60170-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human renal cell carcinoma tissue using TEM1 Monoclonal antibody (60170-1-Ig)

    IHC staining of human renal cell carcinoma using 60170-1-Ig (same clone as 60170-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 60170-1-Ig (TEM1 antibody at dilution of 1:250 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60170-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP51052-1

    Cytometric bead array standard curve of MP51052-1

    Cytometric bead array standard curve of MP51052-1, CD248 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 60170-1-PBS. Detection antibody: 60170-3-PBS. Standard:Ag13334. Range: 6.25-200 ng/mL.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

60170-1-PBS targets TEM1 as part of a matched antibody pair:

MP51052-1: 60170-1-PBS capture and 60170-3-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen TEM1 fusion protein Ag13334 相同性解析による交差性が予測される生物種
Full Name CD248 molecule, endosialin
Calculated molecular weight 757 aa, 81 kDa
Observed molecular weight 150-160 kDa
GenBank accession numberBC051340
Gene symbol TEM1
Gene ID (NCBI) 57124
RRIDAB_10694834
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

TEM1 (Tumor endothelial marker 1), also named as CD248, Endosialin and CD164L1, is a C-type lectin-like domain (CTLD) containing type I transmembrane glycoprotein. It is now considered to be a highly selective marker for activated perivascular and stromal cells, detected in most cancers and at least some inflammatory disorders. CD248 plays a role in tumor angiogenesis. It is a potential diagnostic tool and therapeutic target of inflammatory and malignant disease. Two isoforms of human TEM1 exist. The calculated molecular weights of the two isoforms are 81 kDa and 46 kDa, respectively. Native TEM1 can be glycosylated, and the glycosylated form has a larger apparent molecular weight than 81 kDa.