TERF2 Monoclonal antibody, PBS Only

Name: TERF2 Monoclonal antibody, PBS Only
Brand: Proteintech
SKU: 66893-1-PBS

TERF2 Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

5B1E1

Review Product

Cat no : 66893-1-PBS

Synonyms

Telomeric DNA binding protein, TERF2, TRBF2, TRF2, TTAGGG repeat binding factor 2

Validation Data Gallery

Filter:

AllWBIHC

WB analysis of MCF-7 using 66893-1-Ig (same clone as 66893-1-PBS)
MCF-7 cells were subjected to SDS PAGE followed by western blot with 66893-1-Ig (TERF2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66893-1-PBS in a different storage buffer formulation.
WB analysis of HEK-293 using 66893-1-Ig (same clone as 66893-1-PBS)
HEK-293 cells were subjected to SDS PAGE followed by western blot with 66893-1-Ig (TERF2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66893-1-PBS in a different storage buffer formulation.
WB analysis of Jurkat using 66893-1-Ig (same clone as 66893-1-PBS)
Jurkat cells were subjected to SDS PAGE followed by western blot with 66893-1-Ig (TERF2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66893-1-PBS in a different storage buffer formulation.
WB analysis of 4T1 using 66893-1-Ig (same clone as 66893-1-PBS)
4T1 cells were subjected to SDS PAGE followed by western blot with 66893-1-Ig (TERF2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66893-1-PBS in a different storage buffer formulation.
WB analysis of Daudi using 66893-1-Ig (same clone as 66893-1-PBS)
Daudi cells were subjected to SDS PAGE followed by western blot with 66893-1-Ig (TERF2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66893-1-PBS in a different storage buffer formulation.
WB analysis of HSC-T6 using 66893-1-Ig (same clone as 66893-1-PBS)
HSC-T6 cells were subjected to SDS PAGE followed by western blot with 66893-1-Ig (TERF2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66893-1-PBS in a different storage buffer formulation.
WB analysis of HT-29 using 66893-1-Ig (same clone as 66893-1-PBS)
HT-29 cells were subjected to SDS PAGE followed by western blot with 66893-1-Ig (TERF2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66893-1-PBS in a different storage buffer formulation.
WB analysis of Jurkat using 66893-1-Ig (same clone as 66893-1-PBS)
Jurkat cells were subjected to SDS PAGE followed by western blot with 66893-1-Ig (TERF2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66893-1-PBS in a different storage buffer formulation.
WB analysis of K-562 using 66893-1-Ig (same clone as 66893-1-PBS)
K-562 cells were subjected to SDS PAGE followed by western blot with 66893-1-Ig (TERF2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66893-1-PBS in a different storage buffer formulation.
WB analysis of HSC-T6 using 66893-1-Ig (same clone as 66893-1-PBS)
HSC-T6 cells were subjected to SDS PAGE followed by western blot with 66893-1-Ig (TERF2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66893-1-PBS in a different storage buffer formulation.
WB analysis of NIH/3T3 using 66893-1-Ig (same clone as 66893-1-PBS)
NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66893-1-Ig (TERF2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66893-1-PBS in a different storage buffer formulation.
IHC staining of human gliomas using 66893-1-Ig (same clone as 66893-1-PBS)
Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 66893-1-Ig (TERF2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66893-1-PBS in a different storage buffer formulation.
IHC staining of human gliomas using 66893-1-Ig (same clone as 66893-1-PBS)
Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 66893-1-Ig (TERF2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66893-1-PBS in a different storage buffer formulation.
IHC staining of rat brain using 66893-1-Ig (same clone as 66893-1-PBS)
Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 66893-1-Ig (TERF2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66893-1-PBS in a different storage buffer formulation.
IHC staining of rat brain using 66893-1-Ig (same clone as 66893-1-PBS)
Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 66893-1-Ig (TERF2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66893-1-PBS in a different storage buffer formulation.
IHC staining of mouse brain using 66893-1-Ig (same clone as 66893-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66893-1-Ig (TERF2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66893-1-PBS in a different storage buffer formulation.
IHC staining of mouse brain using 66893-1-Ig (same clone as 66893-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66893-1-Ig (TERF2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66893-1-PBS in a different storage buffer formulation.

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Cat No. 66893-1-PBS

Size/Concentration

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Tested Applications

Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66893-1-PBS targets TERF2 in WB, IHC, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity	Human, mouse, rat
Host / Isotype	Mouse / IgG1
Class	Monoclonal
Type	Antibody
Immunogen	TERF2 fusion protein Ag28513 相同性解析による交差性が予測される生物種
Full Name	telomeric repeat binding factor 2
Calculated molecular weight	56 kDa
Observed molecular weight	60-65.32-35 kDa
GenBank accession number	BC024890
Gene symbol	TERF2
Gene ID (NCBI)	7014
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
Storage Buffer	PBS Only
Storage Conditions	Store at -80°C.

Background Information

TERF2, also named as TRF2 and Telomeric repeat-binding factor 2, is a 542 amino acid protein, which contains 1 HTH myb-type DNA-binding domain and localizes in the Nucleus. TRF2 binds the telomeric double-stranded 5'-TTAGGG-3' repeat and plays a central role in telomere maintenance and protection against end-to-end fusion of chromosomes. TERF2 is a component of the shelterin complex (telosome) that is involved in the regulation of telomere length and protection. TERF2 together with DCLRE1B/Apollo, is required to control the amount of DNA topoisomerase (TOP1, TOP2A and TOP2B), which is needed for telomere replication during fork passage and prevent aberrant telomere topology. TERF2 recruits TERF2IP/RAP1 to telomeres, thereby participating in to repressing homology-directed repair (HDR), which can affect telomere length. TERF2 exists some isoforms with MV 60 kDa and 32 kDa.

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

TERF2 Monoclonal antibody, PBS Only

TERF2 Monoclonal Antibody for WB, IHC, Indirect ELISA

Validation Data Gallery

WB analysis of MCF-7 using 66893-1-Ig (same clone as 66893-1-PBS)

WB analysis of HEK-293 using 66893-1-Ig (same clone as 66893-1-PBS)

WB analysis of Jurkat using 66893-1-Ig (same clone as 66893-1-PBS)

WB analysis of 4T1 using 66893-1-Ig (same clone as 66893-1-PBS)

WB analysis of Daudi using 66893-1-Ig (same clone as 66893-1-PBS)

WB analysis of HSC-T6 using 66893-1-Ig (same clone as 66893-1-PBS)

WB analysis of HT-29 using 66893-1-Ig (same clone as 66893-1-PBS)

WB analysis of Jurkat using 66893-1-Ig (same clone as 66893-1-PBS)

WB analysis of K-562 using 66893-1-Ig (same clone as 66893-1-PBS)

WB analysis of HSC-T6 using 66893-1-Ig (same clone as 66893-1-PBS)

WB analysis of NIH/3T3 using 66893-1-Ig (same clone as 66893-1-PBS)

IHC staining of human gliomas using 66893-1-Ig (same clone as 66893-1-PBS)