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KD/KO Validated

TESC Polyclonal antibody

Name: TESC Polyclonal antibody
Brand: Proteintech
SKU: 11125-1-AP

TESC Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 11125-1-AP

Synonyms

TSC, Tescalcin, CHP3

Validation Data Gallery

Filter:

AllWBIHCIF/ICC

WB analysis using 11125-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 11125-1-AP (TESC antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 11125-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 11125-1-AP (TESC antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
IHC staining of human colon cancer using 11125-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11125-1-AP (TESC antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon cancer using 11125-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11125-1-AP (TESC antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human pancreas cancer using 11125-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 11125-1-AP (TESC antibody) at dilution of 1:50 (under 10x lens).
IHC staining of mouse brain using 11125-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11125-1-AP (TESC antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse brain using 11125-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11125-1-AP (TESC antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of A549 using 11125-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed A549 cells using 11125-1-AP (TESC antibody) at dilution of 1:50 and CoraLite488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Cat No. 11125-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	mouse brain tissue, mouse cerebellum tissue, HeLa cells, rat brain tissue
Positive IHC detected in	human colon cancer tissue, human pancreas cancer tissue, mouse brain tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	A549 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 2 publications below
WB	See 6 publications below
IHC	See 8 publications below

Product Information

11125-1-AP targets TESC in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	TESC fusion protein Ag1596 相同性解析による交差性が予測される生物種
Full Name	tescalcin
Calculated molecular weight	25 kDa
Observed molecular weight	25-30 kDa
GenBank accession number	BC015221
Gene symbol	TESC
Gene ID (NCBI)	54997
RRID	AB_2201727
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for TESC antibody 11125-1-AP	Download protocol
IHC protocol for TESC antibody 11125-1-AP	Download protocol
IF protocol for TESC antibody 11125-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	IHC	Genome Biol Long non-coding RNA ROR decoys gene-specific histone methylation to promote tumorigenesis. Authors - Jiayan Fan View Article
mouse	WB	J Neurosci CHP1-Mediated NHE1 Biosynthetic Maturation Is Required for Purkinje Cell Axon Homeostasis. Authors - Liu Ye Y View Article
human	WB,IHC	Cancer Commun (Lond) Tescalcin is an unfavorable prognosis factor that regulats cell proliferation and survival in hepatocellular carcinoma patients. Authors - Zhong-Guo Zhou KD Validated View Article
human	IHC	Front Oncol The Expression Profile, Clinical Application and Potential Tumor Suppressing Mechanism of hsa_circ_0001675 in Head and Neck Carcinoma. Authors - Yujie Cao View Article
human	WB	Oncol Rep Differential expression of tescalcin by modification of promoter methylation controls cell survival in gastric cancer cells. Authors - Tae Woo Kim View Article
human	IHC	BMC Cancer Tescalcin promotes highly invasive papillary thyroid microcarcinoma by regulating FOS/ERK signaling pathway. Authors - Xiuhe Zou View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

TESC Polyclonal antibody

TESC Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Validation Data Gallery

WB analysis using 11125-1-AP

WB analysis of HeLa using 11125-1-AP

IHC staining of human colon cancer using 11125-1-AP

IHC staining of human colon cancer using 11125-1-AP

IHC staining of human pancreas cancer using 11125-1-AP

IHC staining of mouse brain using 11125-1-AP

IHC staining of mouse brain using 11125-1-AP

IF Staining of A549 using 11125-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

Long non-coding RNA ROR decoys gene-specific histone methylation to promote tumorigenesis.

CHP1-Mediated NHE1 Biosynthetic Maturation Is Required for Purkinje Cell Axon Homeostasis.

Tescalcin is an unfavorable prognosis factor that regulats cell proliferation and survival in hepatocellular carcinoma patients.

The Expression Profile, Clinical Application and Potential Tumor Suppressing Mechanism of hsa_circ_0001675 in Head and Neck Carcinoma.

Differential expression of tescalcin by modification of promoter methylation controls cell survival in gastric cancer cells.

Tescalcin promotes highly invasive papillary thyroid microcarcinoma by regulating FOS/ERK signaling pathway.