Validation Data Gallery
Tested Applications
| Positive WB detected in | A431 cells, HL-60 cells, HEK-293 cells, HeLa cells, HepG2 cells, K-562 cells |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
| Positive FC (Intra) detected in | HeLa cells, RAW 264.7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 14 publications below |
| WB | See 56 publications below |
| IHC | See 11 publications below |
| IF | See 14 publications below |
| CoIP | See 3 publications below |
| ChIP | See 5 publications below |
Product Information
21207-1-AP targets TET2 in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, goat, sheep |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | TET2 fusion protein Ag15583 相同性解析による交差性が予測される生物種 |
| Full Name | tet oncogene family member 2 |
| Calculated molecular weight | 2002 aa, 224 kDa |
| Observed molecular weight | 130 kDa, 250 kDa |
| GenBank accession number | BC150180 |
| Gene Symbol | TET2 |
| Gene ID (NCBI) | 54790 |
| RRID | AB_10734584 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q6N021 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
TET2 is an enzyme which converts 5-methylcytosine (5-mC) to 5-hydroxymethylcytosine (5-hmC) in DNA and is important for normal myelopoiesis. TET2 mutations are frequently observed in myeloid malignancies, including myeloproliferative neoplasms. TET2 is broadly expressed in a variety of tissues. In healthy tissues, TET2 expression was shown to be elevated in hematopoietic cells with highest expression in granulocytes. Three isoforms of TET2 exists due to the alternative splicing. 220-250 kDa bands represent the full-length isoform; 130-133 kDa bands represent the nuclear isoforms of TET2. (PMID: 24615693) This antibody recognizes all three isoforms of TET2.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for TET2 antibody 21207-1-AP | Download protocol |
| IHC protocol for TET2 antibody 21207-1-AP | Download protocol |
| IF protocol for TET2 antibody 21207-1-AP | Download protocol |
| IP protocol for TET2 antibody 21207-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Adv Sci (Weinh) m6A-Modified circTET2 Interacting with HNRNPC Regulates Fatty Acid Oxidation to Promote the Proliferation of Chronic Lymphocytic Leukemia | ||
Sci Adv The chromosomal protein SMCHD1 regulates DNA methylation and the 2c-like state of embryonic stem cells by antagonizing TET proteins. | ||
Sci Adv Reprogramming of DNA methylation at NEUROD2-bound sequences during cortical neuron differentiation. | ||
Nat Commun Redirected nuclear glutamate dehydrogenase supplies Tet3 with α-ketoglutarate in neurons. | ||
J Exp Med TGF-β signaling controls Foxp3 methylation and T reg cell differentiation by modulating Uhrf1 activity. | ||
JACC Basic Transl Sci Hypermethylation of the Micro-RNA 145 Promoter Is the Key Regulator for NLRP3 Inflammasome-Induced Activation and Plaque Formation. |