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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

TET3 Polyclonal antibody

TET3 Polyclonal Antibody for IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 22612-1-AP

Synonyms

tet oncogene family member 3, Methylcytosine dioxygenase TET3, KIAA0401, EC:1.14.11.80

Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of mouse brain tissue using TET3 Polyclonal antibody (22612-1-AP)

    IHC staining of mouse brain using 22612-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 22612-1-AP (TET3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using TET3 Polyclonal antibody (22612-1-AP)

    IF Staining of U2OS using 22612-1-AP

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using TET3 antibody (22612-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Tested Applications

Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inU2OS cells

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

22612-1-AP targets TET3 in IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TET3 fusion protein Ag18379 相同性解析による交差性が予測される生物種
Full Name tet oncogene family member 3
Calculated molecular weight 1660 aa, 179 kDa
GenBank accession numberBC022243
Gene symbol TET3
Gene ID (NCBI) 200424
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Ten-eleven translocation protein 3 (TET3) belongs to the family of ten-eleven translocations (TETs) which induce DNA demethylation and gene regulation at the epigenetic level by converting 5-methylcytosine (5mC) to 5-hydroxymethylcytosine (5hmC) (PMID: 31656201). In ovarian cancer, TET3 is reported as an oncogene or tumor suppressor during tumorigenesis and overexpressed in ovarian cancer tissues (PMID: 27141829).

Protocols

Product Specific Protocols
IHC protocol for TET3 antibody 22612-1-APDownload protocol
IF protocol for TET3 antibody 22612-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols