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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

TEX13A Polyclonal antibody

TEX13A Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 18888-1-AP

Synonyms

testis expressed 13A, TEX13A

Validation Data Gallery

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  • Western Blot (WB) analysis of HL-60 cells using TEX13A Polyclonal antibody (18888-1-AP)

    WB analysis of HL-60 using 18888-1-AP

    HL-60 cells were subjected to SDS PAGE followed by western blot with 18888-1-AP (TEX13A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse spermatophore tissue using TEX13A Polyclonal antibody (18888-1-AP)

    WB analysis of mouse spermatophore using 18888-1-AP

    mouse spermatophore tissue were subjected to SDS PAGE followed by western blot with 18888-1-AP (TEX13A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of PC-3 cells using TEX13A Polyclonal antibody (18888-1-AP)

    WB analysis of PC-3 using 18888-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 18888-1-AP (TEX13A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of K-562 cells using TEX13A Polyclonal antibody (18888-1-AP)

    WB analysis of K-562 using 18888-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 18888-1-AP (TEX13A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human testis tissue using TEX13A Polyclonal antibody (18888-1-AP)

    IHC staining of human testis using 18888-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 18888-1-AP (TEX13A antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using TEX13A Polyclonal antibody (18888-1-AP)

    IHC staining of human testis using 18888-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 18888-1-AP (TEX13A antibody) at dilution of 1:100 (under 40x lens).

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Tested Applications

Positive WB detected inHL-60 cells, PC-3 cells, K-562 cells, mouse spermatophore tissue
Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

The immunogen of 18888-1-AP is TEX13A Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TEX13A fusion protein Ag13468 相同性解析による交差性が予測される生物種
Full Name testis expressed 13A
Calculated molecular weight 409 aa, 46 kDa
Observed molecular weight 46 kDa
GenBank accession numberBC042547
Gene symbol TEX13A
Gene ID (NCBI) 56157
RRIDAB_10641998
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for TEX13A antibody 18888-1-APDownload protocol
IHC protocol for TEX13A antibody 18888-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cancer Lett

CDK5RAP3 is a novel super-enhancer-driven gene activated by master TFs and regulates ER-Phagy in neuroblastoma

Authors - Ran Zhuo
View Article