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Validation Data Gallery

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  • Western Blot (WB) analysis of rat testis tissue using TEX14 Monoclonal antibody (67982-1-Ig)

    WB analysis of rat testis using 67982-1-Ig (same clone as 67982-1-PBS)

    rat testis tissue were subjected to SDS PAGE followed by western blot with 67982-1-Ig (TEX14 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67982-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse testis tissue using TEX14 Monoclonal antibody (67982-1-Ig)

    WB analysis of mouse testis using 67982-1-Ig (same clone as 67982-1-PBS)

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 67982-1-Ig (TEX14 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67982-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rabbit testis tissue using TEX14 Monoclonal antibody (67982-1-Ig)

    WB analysis of rabbit testis using 67982-1-Ig (same clone as 67982-1-PBS)

    rabbit testis tissue were subjected to SDS PAGE followed by western blot with 67982-1-Ig (TEX14 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67982-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of PC-3 cells using TEX14 Monoclonal antibody (67982-1-Ig)

    WB analysis of PC-3 using 67982-1-Ig (same clone as 67982-1-PBS)

    PC-3 cells were subjected to SDS PAGE followed by western blot with 67982-1-Ig (TEX14 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67982-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse testis tissue using TEX14 Monoclonal antibody (67982-1-Ig)

    IF Staining of mouse testis using 67982-1-Ig (same clone as 67982-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using TEX14 antibody (67982-1-Ig, Clone: 1A11D6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67982-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse testis tissue using TEX14 Monoclonal antibody (67982-1-Ig)

    IF Staining of mouse testis using 67982-1-Ig (same clone as 67982-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using TEX14 antibody (67982-1-Ig, Clone: 1A11D6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67982-1-PBS in a different storage buffer formulation.

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TEX14 Monoclonal antibody, PBS Only

TEX14 Monoclonal Antibody for WB, IF-P, Indirect ELISA
Cat No. 67982-1-PBS
Clone No.1A11D6

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse, rat, rabbit

Applications

WB, IF-P, Indirect ELISA

Synonyms

SGK307, SGK307, Sugen kinase 307, testis expressed 14, Testis expressed protein 14, Testis expressed sequence 14, TEX14

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67982-1-PBS targets TEX14 in WB, IF-P, Indirect ELISA applications and shows reactivity with Human, mouse, rat, rabbit samples.

Tested Reactivity Human, mouse, rat, rabbit
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen TEX14 fusion protein Ag13143 相同性解析による交差性が予測される生物種
Full Name testis expressed 14
Calculated molecular weight957aa,107 kDa; 1497aa,168 kDa
Observed molecular weight180 kDa
GenBank accession numberBC040526
Gene Symbol TEX14
Gene ID (NCBI) 56155
RRIDAB_2918731
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ8IWB6
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Tex14 is required both for the formation of intercellular bridges during meiosis and for kinetochore-microtubule attachment during mitosis. Intercellular bridges are evolutionarily conserved structures that connect differentiating germ cells and are required for spermatogenesis and male fertility. Tex14 acts by promoting the conversion of midbodies into intercellular bridges via its interaction with CEP55: interaction with CEP55 inhibits the interaction between CEP55 and PDCD6IP/ALIX and TSG101, blocking cell abscission and leading to transform midbodies into intercellular bridges.Tex14 also plays a role during mitosis: recruited to kinetochores by PLK1 during early mitosis and regulates the maturation of the outer kinetochores and microtubule attachment.