TEX15 Polyclonal antibody
TEX15 Polyclonal Antibody for WB, IP, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IP, IHC, ELISA
Conjugate
Unconjugated
Cat no : 24585-1-AP
Synonyms
Validation Data Gallery
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Tested Applications
| Positive WB detected in | rat testis tissue |
| Positive IP detected in | PC-3 cells |
| Positive IHC detected in | mouse testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 2 publications below |
Product Information
The immunogen of 24585-1-AP is TEX15 Fusion Protein expressed in E. coli.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | TEX15 fusion protein Ag20170 相同性解析による交差性が予測される生物種 |
| Full Name | testis expressed 15 |
| Calculated molecular weight | 2789 aa, 315 kDa |
| Observed molecular weight | 150 kDa |
| GenBank accession number | BC141841 |
| Gene symbol | TEX15 |
| Gene ID (NCBI) | 56154 |
| RRID | AB_2879623 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
TEX15 also named as Cancer/testis antigen 42 is a 2789 amino acid protein, which is expressed exclusively in testicular and cancerous tissues and may be involved in the development and metastasis of several different types of tumors. TEX15 exists as a 1279 amino acid protein in the mouse and rat testis. The molecular weight of mouse/rat TEX15 is 150 kDa (PMID: 26959739).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for TEX15 antibody 24585-1-AP | Download protocol |
| IHC protocol for TEX15 antibody 24585-1-AP | Download protocol |
| IP protocol for TEX15 antibody 24585-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Toxicol Lett Downregulation of vdac2 inhibits spermatogenesis via JNK and P53 signalling in mice exposed to cadmium. | ||
Fertil Steril Differential expression of miR-23a/b-3p and its target genes in male patients with subfertility. |
