Transferrin/TF Monoclonal antibody
Transferrin/TF Monoclonal Antibody for WB, IHC, IF-P, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
human
Applications
WB, IHC, IF-P, ELISA
Conjugate
Unconjugated
CloneNo.
2B4C6
Cat no : 66171-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | human plasma, fetal human brain tissue |
| Positive IHC detected in | human liver cancer tissue, human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | human liver tissue, human placenta tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:2000 |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 5 publications below |
Product Information
66171-1-Ig targets Transferrin/TF in WB, IHC, IF-P, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | Transferrin/TF fusion protein Ag11668 相同性解析による交差性が予測される生物種 |
| Full Name | transferrin |
| Calculated molecular weight | 698 aa, 77 kDa |
| Observed molecular weight | 77 kDa |
| GenBank accession number | BC059367 |
| Gene symbol | TF |
| Gene ID (NCBI) | 7018 |
| RRID | AB_2881566 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
What is the tissue specificity of TF?
Transferrin is synthesized mainly in the liver.
What is the calculated molecular weight of TF?
77 kDa
How many iron atoms can bind to TF?
Each of the two lobes of TF has one high-affinity Fe(III) binding site. Since binding of iron and bicarbonate
releases free H+ ions, affinity decreases in acidic conditions, in which TF also remains bound to its receptor.
What is the primary role of TF?
TF serves as a serum metal-binding protein, playing a key role in iron metabolism and delivery for erythropoiesis.
What is the role of TF in human diseases?
TF deficiency results in an exhibition of congenital atransferrinemia (hypotransferrinemia) in which there is a
decrease in hepcidin expression, causing increased iron levels.
Is TF essential for iron delivery?
Congenital atransferrinemia results in an iron overload in some tissues, such as the liver, suggesting that while TF is not essential for iron delivery to all organs, it is needed for iron level regulation.
In what circumstances do TF levels decrease?
Inflammation and iron overload both can cause a decline in TF levels.
How does TF affect hormonal balance?
TF positively regulates expression of hepcidin, a peptide hormone synthesized by the liver.
How does TF deficiency cause increased iron levels?
TF regulates hepcidin, which in turn is a negative regulator of intestinal iron absorption, resulting in TF
deficiency causing both anemia, and, paradoxically, increased iron levels in some tissues.
PMIDs: 23046645, 9635730, 22294463, 24589273
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Transferrin/TF antibody 66171-1-Ig | Download protocol |
| IHC protocol for Transferrin/TF antibody 66171-1-Ig | Download protocol |
| IF protocol for Transferrin/TF antibody 66171-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Death Dis Super-enhancer-driven MLX mediates redox balance maintenance via SLC7A11 in osteosarcoma | ||
Eur Rev Med Pharmacol Sci Decreased serum SIRT6 as a novel predictor of coronary artery disease | ||
Neurochem Int Quantitative iTRAQ-based proteomic analysis of piperine protected cerebral ischemia/reperfusion injury in rat brain. |
