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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TFAM Recombinant antibody (82745-1-RR)

    WB analysis using 82745-1-RR (same clone as 82745-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82745-1-RR (TFAM antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82745-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human intrahepatic cholangiocarcinoma tissue using TFAM Recombinant antibody (82745-1-RR)

    IHC staining of human intrahepatic cholangiocarcinoma using 82745-1-RR (same clone as 82745-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 82745-1-RR (TFAM antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82745-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human intrahepatic cholangiocarcinoma tissue using TFAM Recombinant antibody (82745-1-RR)

    IHC staining of human intrahepatic cholangiocarcinoma using 82745-1-RR (same clone as 82745-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 82745-1-RR (TFAM antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82745-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using TFAM Recombinant antibody (82745-1-RR)

    IF Staining of HepG2 using 82745-1-RR (same clone as 82745-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using TFAM antibody (82745-1-RR, Clone: 4L4 ) at dilution of 1:250 and Multi-rAb CoraLite ® Plus 594-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR004). This data was developed using the same antibody clone with 82745-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82745-1-RR

    Affinity and Kinetic Characterization of 82745-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82745-1-RR against Human TFAM were performed. The affinity constant is 45.7 pM.

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TFAM Recombinant antibody, PBS Only

TFAM Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 82745-1-PBS
Clone No.4L4

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Transcription factor 6-like 2, TCF-6, TCF6, mtTFA, MtTF1

Formulation:  PBS Only
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

82745-1-PBS targets TFAM in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen TFAM fusion protein Ag18299 相同性解析による交差性が予測される生物種
Full Name transcription factor A, mitochondrial
Calculated molecular weight 246 aa, 29 kDa
Observed molecular weight24-25 kDa
GenBank accession numberBC126366
Gene Symbol TFAM
Gene ID (NCBI) 7019
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ00059
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

TFAM, also named as TCF6L2, MtTF1, TCF6, TCF6L1, TCF6L3 and mtTFA, is a mitochondrial transcription factor that is a key activator of mitochondrial transcription as well as a participant in mitochondrial genome replication. It is involved in mitochondrial transcription regulation. TFAM is required for accurate and efficient promoter recognition by the mitochondrial RNA polymerase. It activates transcription by binding immediately upstream of transcriptional start sites. TFAM is able to unwind and bend DNA.