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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

TFAM Recombinant antibody

TFAM Recombinant Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

4L4

Cat no : 82745-1-RR

Synonyms

Transcription factor 6-like 2, TCF-6, TCF6, mtTFA, MtTF1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TFAM Recombinant antibody (82745-1-RR)

    WB analysis using 82745-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82745-1-RR (TFAM antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human intrahepatic cholangiocarcinoma tissue using TFAM Recombinant antibody (82745-1-RR)

    IHC staining of human intrahepatic cholangiocarcinoma using 82745-1-RR

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 82745-1-RR (TFAM antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human intrahepatic cholangiocarcinoma tissue using TFAM Recombinant antibody (82745-1-RR)

    IHC staining of human intrahepatic cholangiocarcinoma using 82745-1-RR

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 82745-1-RR (TFAM antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using TFAM Recombinant antibody (82745-1-RR)

    IF Staining of HepG2 using 82745-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using TFAM antibody (82745-1-RR, Clone: 4L4 ) at dilution of 1:250 and Multi-rAb CoraLite ® Plus 594-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR004).

  • Affinity and Kinetic Characterization of 82745-1-RR

    Affinity and Kinetic Characterization of 82745-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82745-1-RR against Human TFAM were performed. The affinity constant is 45.7 pM.

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Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, MCF-7 cells, Jurkat cells, K-562 cells
Positive IHC detected inhuman intrahepatic cholangiocarcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

82745-1-RR targets TFAM in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen TFAM fusion protein Ag18299 相同性解析による交差性が予測される生物種
Full Name transcription factor A, mitochondrial
Calculated molecular weight 246 aa, 29 kDa
Observed molecular weight24-25 kDa
GenBank accession numberBC126366
Gene symbol TFAM
Gene ID (NCBI) 7019
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TFAM, also named as TCF6L2, MtTF1, TCF6, TCF6L1, TCF6L3 and mtTFA, is a mitochondrial transcription factor that is a key activator of mitochondrial transcription as well as a participant in mitochondrial genome replication. It is involved in mitochondrial transcription regulation. TFAM is required for accurate and efficient promoter recognition by the mitochondrial RNA polymerase. It activates transcription by binding immediately upstream of transcriptional start sites. TFAM is able to unwind and bend DNA.

Protocols

Product Specific Protocols
WB protocol for TFAM antibody 82745-1-RRDownload protocol
IHC protocol for TFAM antibody 82745-1-RRDownload protocol
IF protocol for TFAM antibody 82745-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols