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  • KD/KO Validated

TFAP2A/AP-2 Polyclonal antibody

TFAP2A/AP-2 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 13019-3-AP

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IF/ICC, IP, ChIP, ELISA

TFAP2A, TFAP2A,AP-2, AP-2 transcription factor, AP-2, AP2

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration:  150 μL, 450 ug/ml
20 μL, 450 ug/ml
150 μL, 450 ug/ml

¥67,000/ 150 μL

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約2万点のプロテインテック製品をコスモバイオ社物流センター(国内)に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。


保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換/補填対応を承ります。詳細はこちらをご覧ください。


Tested Applications

Positive WB detected inHepG2 cells, Y79 cells, MCF-7 cells
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13019-3-AP targets TFAP2A/AP-2 in WB, IF/ICC, IP, ChIP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TFAP2A/AP-2 fusion protein Ag4112 相同性解析による交差性が予測される生物種
Full Name transcription factor AP-2 alpha (activating enhancer binding protein 2 alpha)
Calculated molecular weight 431 aa, 47 kDa
Observed molecular weight 47 kDa
GenBank accession numberBC017754
Gene Symbol TFAP2A/AP-2
Gene ID (NCBI) 7020
RRIDAB_2199414
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP05549
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The activator protein-2 (AP-2) family of transcription factors comprises five 52-kDa isoforms (AP-2α, AP-2β, AP-2γ, AP-2δ, and AP-2ε), which share a common structure: a proline/glutamine-rich transactivation domain in the N-terminal region and a helix-span-helix domain in the C-terminal region, which mediates dimerization and site-specific DNA binding. Depending on the cellular context, the AP-2 transcription factors are individually associated either with cell differentiation and development or with cancer progression/regression (PMID: 21966377). TFAP2A (AP-2-alpha) is the only AP-2 protein required for early morphogenesis of the lens vesicle. Together with the CITED2 coactivator, stimulates the PITX2 P1 promoter transcription activation (PMID: 11694877).

Protocols

Product Specific Protocols
WB protocol for TFAP2A/AP-2 antibody 13019-3-APDownload protocol
IF protocol for TFAP2A/AP-2 antibody 13019-3-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle

Oncol Rep

Regulation of DEK expression by AP-2α and methylation level of DEK promoter in hepatocellular carcinoma.

Authors - Ming-Xu Qiao
human, mouseWB, ChIP

Acta Pharmacol Sin

Hepatitis B virus X protein promotes human hepatoma cell growth via upregulation of transcription factor AP2α and sphingosine kinase 1.

Authors - Zhan-Ping Lu
  • KD Validated
ChIP

Cell Death Dis

TFAP2A potentiates lung adenocarcinoma metastasis by a novel miR-16 family/TFAP2A/PSG9/TGF-β signaling pathway.

Authors - Yanlu Xiong
humanWB

Cell Death Dis

The m6A modification-mediated OGDHL exerts a tumor suppressor role in ccRCC by downregulating FASN to inhibit lipid synthesis and ERK signaling

Authors - Jian Shi
humanChIP

Int Immunopharmacol

miR-1293 suppresses osteosarcoma progression by modulating drug sensitivity in response to cisplatin treatment

Authors - Tingxuan Wang
humanChIP

Commun Biol

Macroscopic inhibition of DNA damage repair pathways by targeting AP-2α with LEI110 eradicates hepatocellular carcinoma

Authors - Chenchen Wang
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