Validation Data Gallery
Tested Applications
| Positive WB detected in | HepG2 cells, Y79 cells, MCF-7 cells |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 5 publications below |
| IP | See 1 publications below |
| ChIP | See 5 publications below |
Product Information
13019-3-AP targets TFAP2A/AP-2 in WB, IF/ICC, IP, ChIP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | TFAP2A/AP-2 fusion protein Ag4112 相同性解析による交差性が予測される生物種 |
| Full Name | transcription factor AP-2 alpha (activating enhancer binding protein 2 alpha) |
| Calculated molecular weight | 431 aa, 47 kDa |
| Observed molecular weight | 47 kDa |
| GenBank accession number | BC017754 |
| Gene Symbol | TFAP2A/AP-2 |
| Gene ID (NCBI) | 7020 |
| RRID | AB_2199414 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P05549 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
The activator protein-2 (AP-2) family of transcription factors comprises five 52-kDa isoforms (AP-2α, AP-2β, AP-2γ, AP-2δ, and AP-2ε), which share a common structure: a proline/glutamine-rich transactivation domain in the N-terminal region and a helix-span-helix domain in the C-terminal region, which mediates dimerization and site-specific DNA binding. Depending on the cellular context, the AP-2 transcription factors are individually associated either with cell differentiation and development or with cancer progression/regression (PMID: 21966377). TFAP2A (AP-2-alpha) is the only AP-2 protein required for early morphogenesis of the lens vesicle. Together with the CITED2 coactivator, stimulates the PITX2 P1 promoter transcription activation (PMID: 11694877).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for TFAP2A/AP-2 antibody 13019-3-AP | Download protocol |
| IF protocol for TFAP2A/AP-2 antibody 13019-3-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Oncol Rep Regulation of DEK expression by AP-2α and methylation level of DEK promoter in hepatocellular carcinoma. | ||
Acta Pharmacol Sin Hepatitis B virus X protein promotes human hepatoma cell growth via upregulation of transcription factor AP2α and sphingosine kinase 1.
| ||
Cell Death Dis TFAP2A potentiates lung adenocarcinoma metastasis by a novel miR-16 family/TFAP2A/PSG9/TGF-β signaling pathway. | ||
Cell Death Dis The m6A modification-mediated OGDHL exerts a tumor suppressor role in ccRCC by downregulating FASN to inhibit lipid synthesis and ERK signaling | ||
Int Immunopharmacol miR-1293 suppresses osteosarcoma progression by modulating drug sensitivity in response to cisplatin treatment | ||
Commun Biol Macroscopic inhibition of DNA damage repair pathways by targeting AP-2α with LEI110 eradicates hepatocellular carcinoma |