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Validation Data Gallery

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  • Western Blot (WB) analysis of L02 cells using TFAP2A/AP-2 Monoclonal antibody (67076-1-Ig)

    WB analysis of L02 using 67076-1-Ig (same clone as 67076-1-PBS)

    L02 cells were subjected to SDS PAGE followed by western blot with 67076-1-Ig (TFAP2A/AP-2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67076-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using TFAP2A/AP-2 Monoclonal antibody (67076-1-Ig)

    WB analysis of HepG2 using 67076-1-Ig (same clone as 67076-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 67076-1-Ig (TFAP2A/AP-2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67076-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of MCF-7 cells using TFAP2A/AP-2 Monoclonal antibody (67076-1-Ig)

    WB analysis of MCF-7 using 67076-1-Ig (same clone as 67076-1-PBS)

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 67076-1-Ig (TFAP2A/AP-2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67076-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HSC-T6 cells using TFAP2A/AP-2 Monoclonal antibody (67076-1-Ig)

    WB analysis of HSC-T6 using 67076-1-Ig (same clone as 67076-1-PBS)

    HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67076-1-Ig (TFAP2A/AP-2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67076-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using TFAP2A/AP-2 Monoclonal antibody (67076-1-Ig)

    WB analysis of HeLa using 67076-1-Ig (same clone as 67076-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 67076-1-Ig (TFAP2A/AP-2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67076-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using TFAP2A/AP-2 Monoclonal antibody (67076-1-Ig)

    IHC staining of human breast cancer using 67076-1-Ig (same clone as 67076-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67076-1-Ig (TFAP2A/AP-2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67076-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using TFAP2A/AP-2 Monoclonal antibody (67076-1-Ig)

    IHC staining of human breast cancer using 67076-1-Ig (same clone as 67076-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67076-1-Ig (TFAP2A/AP-2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67076-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of A431 cells using TFAP2A/AP-2 Monoclonal antibody (67076-1-Ig)

    FC experiment of A431 using 67076-1-Ig (same clone as 67076-1-PBS)

    1X10^6 A431 cells were intracellularly stained with 0.4 ug Anti-Human TFAP2A/AP-2 (67076-1-Ig, Clone:1B12F11) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 67076-1-PBS in a different storage buffer formulation.

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TFAP2A/AP-2 Monoclonal antibody, PBS Only

TFAP2A/AP-2 Monoclonal Antibody for WB, IHC, FC (Intra), ELISA
Cat No. 67076-1-PBS
Clone No.1B12F11

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, FC (Intra), ELISA

Synonyms

TFAP2A, TFAP2A,AP-2, AP-2, AP2, AP 2alpha

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67076-1-PBS targets TFAP2A/AP-2 in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen TFAP2A/AP-2 fusion protein Ag4112 相同性解析による交差性が予測される生物種
Full Name transcription factor AP-2 alpha (activating enhancer binding protein 2 alpha)
Calculated molecular weight 431 aa, 47 kDa
Observed molecular weight 47 kDa
GenBank accession numberBC017754
Gene Symbol TFAP2A/AP-2
Gene ID (NCBI) 7020
RRIDAB_2882384
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP05549
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

The activator protein-2 (AP-2) family of transcription factors comprises five 52-kDa isoforms (AP-2α, AP-2β, AP-2γ, AP-2δ, and AP-2ε), which share a common structure: a proline/glutamine-rich transactivation domain in the N-terminal region and a helix-span-helix domain in the C-terminal region, which mediates dimerization and site-specific DNA binding. Depending on the cellular context, the AP-2 transcription factors are individually associated either with cell differentiation and development or with cancer progression/regression. [PMID:21966377] TFAP2A (AP-2-alpha) is the only AP-2 protein required for early morphogenesis of the lens vesicle. Together with the CITED2 coactivator, stimulates the PITX2 P1 promoter transcription activation [PMID:11694877]