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KD/KO Validated

TFAP2C Polyclonal antibody

Name: TFAP2C Polyclonal antibody
Brand: Proteintech
SKU: 14572-1-AP

TFAP2C Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IP, chIP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 14572-1-AP

Synonyms

ap 2gamma, AP2 GAMMA, ERF1, hAP 2g, TFAP2C, TFAP2G, Transcription factor ERF 1

Validation Data Gallery

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AllWBIPIHC

WB analysis of HeLa using 14572-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 14572-1-AP (TFAP2C antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of human placenta using 14572-1-AP
human placenta tissue were subjected to SDS PAGE followed by western blot with 14572-1-AP (TFAP2C antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of MCF-7 using 14572-1-AP
MCF7 cells were subjected to SDS PAGE followed by western blot with 14572-1-AP (TFAP2C antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of HeLa using 14572-1-AP
IP result of anti-TFAP2C (IP:14572-1-AP, 4ug; Detection:14572-1-AP 1:1000) with HeLa cells lysate 2800ug.
IHC staining of human breast cancer using 14572-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14572-1-AP (TFAP2C Antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 14572-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer using 14572-1-AP (TFAP2C antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human breast cancer using 14572-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer using 14572-1-AP (TFAP2C antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human breast cancer using 14572-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14572-1-AP (TFAP2C Antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Cat No. 14572-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	HeLa cells, human placenta tissue, MCF-7 cells
Positive IP detected in	HeLa cells
Positive IHC detected in	human breast cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2400
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 2 publications below
WB	See 6 publications below
IHC	See 3 publications below
ChIP	See 3 publications below

Product Information

14572-1-AP targets TFAP2C in WB, IHC, IP, chIP, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	TFAP2C fusion protein Ag5648 相同性解析による交差性が予測される生物種
Full Name	transcription factor AP-2 gamma (activating enhancer binding protein 2 gamma)
Calculated molecular weight	49 kDa
Observed molecular weight	50 kDa
GenBank accession number	BC035664
Gene symbol	TFAP2C
Gene ID (NCBI)	7022
RRID	AB_2271424
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for TFAP2C antibody 14572-1-AP	Download protocol
IHC protocol for TFAP2C antibody 14572-1-AP	Download protocol
IP protocol for TFAP2C antibody 14572-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB,ChIP	Mol Cancer CircIL4R activates the PI3K/AKT signaling pathway via the miR-761/TRIM29/PHLPP1 axis and promotes proliferation and metastasis in colorectal cancer. Authors - Tao Jiang KD Validated View Article
human	WB	Oncogene Abrogation of glutathione peroxidase-1 drives EMT and chemoresistance in pancreatic cancer by activating ROS-mediated Akt/GSK3β/Snail signaling. Authors - Qingcai Meng View Article
human	chIP	Oncogene TFAP2C regulates carbonic anhydrase XII in human breast cancer. Authors - Christopher M Franke View Article
human	WB	Front Oncol TFAP2C-Mediated lncRNA PCAT1 Inhibits Ferroptosis in Docetaxel-Resistant Prostate Cancer Through c-Myc/miR-25-3p/SLC7A11 Signaling. Authors - Xingkang Jiang View Article
human	WB,IHC	Cancers (Basel) TFAP2C Knockdown Sensitizes Bladder Cancer Cells to Cisplatin Treatment via Regulation of EGFR and NF-κB Authors - Ji Xing KD Validated View Article
human	IHC	Nat Commun Single-cell multi-omics analysis of human testicular germ cell tumor reveals its molecular features and microenvironment Authors - Xiaojian Lu View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

TFAP2C Polyclonal antibody

TFAP2C Polyclonal Antibody for WB, IP, IHC, ELISA

Validation Data Gallery

WB analysis of HeLa using 14572-1-AP

WB analysis of human placenta using 14572-1-AP

WB analysis of MCF-7 using 14572-1-AP

IP experiment of HeLa using 14572-1-AP

IHC staining of human breast cancer using 14572-1-AP

IHC staining of human breast cancer using 14572-1-AP

IHC staining of human breast cancer using 14572-1-AP

IHC staining of human breast cancer using 14572-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

CircIL4R activates the PI3K/AKT signaling pathway via the miR-761/TRIM29/PHLPP1 axis and promotes proliferation and metastasis in colorectal cancer.

Abrogation of glutathione peroxidase-1 drives EMT and chemoresistance in pancreatic cancer by activating ROS-mediated Akt/GSK3β/Snail signaling.

TFAP2C regulates carbonic anhydrase XII in human breast cancer.

TFAP2C-Mediated lncRNA PCAT1 Inhibits Ferroptosis in Docetaxel-Resistant Prostate Cancer Through c-Myc/miR-25-3p/SLC7A11 Signaling.

TFAP2C Knockdown Sensitizes Bladder Cancer Cells to Cisplatin Treatment via Regulation of EGFR and NF-κB

Single-cell multi-omics analysis of human testicular germ cell tumor reveals its molecular features and microenvironment