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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using TFAP2D Polyclonal antibody (18835-1-AP)

    WB analysis of HeLa using 18835-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 18835-1-AP (TFAP2D Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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TFAP2D Polyclonal antibody

TFAP2D Polyclonal Antibody for WB, ELISA
Cat No. 18835-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

AP2 delta, TFAP2BL1, TFAP2D

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

18835-1-AP targets TFAP2D in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name transcription factor AP-2 delta (activating enhancer binding protein 2 delta)
Calculated molecular weight 50 kDa
Observed molecular weight 47-52 kDa
GenBank accession numberBC166653
Gene Symbol TFAP2D
Gene ID (NCBI) 83741
RRIDAB_2878562
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ7Z6R9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TFAP2D, also named as TFAP2BL1, belongs to the AP-2 family. TFAP2D is a sequence-specific DNA-binding protein that interacts with inducible viral and cellular enhancer elements to regulate transcription of selected genes. AP-2 factors bind to the consensus sequence 5'-GCCNNNGGC-3' and activate genes involved in a large spectrum of important biological functions including proper eye, face, body wall, limb and neural tube development. They also suppress a number of genes including MCAM/MUC18, C/EBP alpha and MYC. It is highly expressed in adult thymus, prostate, small intestine, skeletal muscle, placenta, brain, and testis tissues.

Protocols

Product Specific Protocols
WB protocol for TFAP2D antibody 18835-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols