Validation Data Gallery
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Tested Applications
| Positive WB detected in | A549 cells, HeLa cells, Jurkat cells, NCI-H1299 cells |
| Positive IP detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 4 publications below |
| IHC | See 1 publications below |
| ChIP | See 1 publications below |
Product Information
12017-1-AP targets TFAP4 in WB, IP, IHC, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | TFAP4 fusion protein Ag2633 相同性解析による交差性が予測される生物種 |
| Full Name | transcription factor AP-4 (activating enhancer binding protein 4) |
| Calculated molecular weight | 338 aa, 39 kDa |
| Observed molecular weight | 39-42 kDa |
| GenBank accession number | BC010576 |
| Gene Symbol | TFAP4 |
| Gene ID (NCBI) | 7023 |
| RRID | AB_2199571 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q01664 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for TFAP4 antibody 12017-1-AP | Download protocol |
| IP protocol for TFAP4 antibody 12017-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Front Immunol Targeting NK-1R attenuates renal fibrosis via modulating inflammatory responses and cell fate in chronic kidney disease | ||
J Agric Food Chem Cucurbitacin E Triggers Cellular Senescence in Colon Cancer Cells via Regulating the miR-371b-5p/TFAP4 Signaling Pathway. | ||
J Agric Food Chem Cucurbitacin E Chemosensitizes Colorectal Cancer Cells via Mitigating TFAP4/Wnt/β-Catenin Signaling. | ||
Invest Ophthalmol Vis Sci A Novel Small Peptide H-KI20 Inhibits Retinal Neovascularization Through the JNK/ATF2 Signaling Pathway. |