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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

TFAP4 Recombinant antibody

TFAP4 Recombinant Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

240031D5

Cat no : 83196-4-RR

Synonyms

AP 4, bHLHc41, TFAP4, Transcription factor AP 4

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TFAP4 Recombinant antibody (83196-4-RR)

    WB analysis using 83196-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83196-4-RR (TFAP4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using TFAP4 Recombinant antibody (83196-4-RR)

    IF Staining of HeLa using 83196-4-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using TFAP4 antibody (83196-4-RR, Clone: 240031D5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 83196-4-RR

    Affinity and Kinetic Characterization of 83196-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83196-4-RR against Human TFAP4 were performed. The affinity constant is below 1 pM.

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Tested Applications

Positive WB detected inHeLa cells, Jurkat cells, A549 cells, NCI-H1299 cells
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83196-4-RR is TFAP4 Fusion Protein expressed in E. coli.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen TFAP4 fusion protein Ag2633 相同性解析による交差性が予測される生物種
Full Name transcription factor AP-4 (activating enhancer binding protein 4)
Calculated molecular weight 338 aa, 39 kDa
Observed molecular weight39-42 kDa
GenBank accession numberBC010576
Gene symbol TFAP4
Gene ID (NCBI) 7023
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for TFAP4 antibody 83196-4-RRDownload protocol
IF protocol for TFAP4 antibody 83196-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols