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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using TFDP2 Recombinant antibody (84408-4-RR)

    IF Staining of U2OS using 84408-4-RR

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using TFDP2 antibody (84408-4-RR, Clone: 241457A12 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Flow cytometry (FC) experiment of A431 cells using TFDP2 Recombinant antibody (84408-4-RR)

    FC experiment of A431 using 84408-4-RR

    1x10^6 A431 cells were intracellularly stained with 0.25 ug TFDP2 Recombinant antibody (84408-4-RR, Clone:241457A12) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

  • Affinity and Kinetic Characterization of 84408-4-RR

    Affinity and Kinetic Characterization of 84408-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84408-4-RR against Human TFDP2 were performed. The affinity constant is below 1 pM.

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TFDP2 Recombinant antibody

TFDP2 Recombinant Antibody for IF/ICC, FC (Intra), ELISA
Cat No. 84408-4-RR
Clone No.241457A12

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra), ELISA

Synonyms

Transcription factor Dp-2, DP2, Dp 2, 241457A12

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration: 

-/ -

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Tested Applications

Positive IF/ICC detected inU2OS cells
Positive FC (Intra) detected inA431 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84408-4-RR targets TFDP2 in IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen TFDP2 fusion protein Ag2054 相同性解析による交差性が予測される生物種
Full Name transcription factor Dp-2 (E2F dimerization partner 2)
Calculated molecular weight 446 aa, 49 kDa
GenBank accession numberBC021113
Gene Symbol TFDP2
Gene ID (NCBI) 7029
RRIDAB_3671937
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ14188
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TFDP2, also known as E2FDP2, is a coactivator of E2F factors and involves in EGF or IFN-gamma-mediated effects by enhancing or reducing active heterodimeric complexes. The DP2/E2F complex functions in the control of cell-cycle progression from G1 to S phase. The E2F1/DP complex appears to mediate both cell proliferation and apoptosis [PMID:16360038]. Binds DNA cooperatively with E2F family members through the E2 recognition site, 5'-TTTC[CG]CGC-3', found in the promoter region of several genes whose products are involved in cell cycle regulation or DNA replication [PMID:16721044].

Protocols

Product Specific Protocols
IF protocol for TFDP2 antibody 84408-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols