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- KD/KO Validated
TFE3 Polyclonal antibody
TFE3 Polyclonal Antibody for WB, IP, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF, IP, ChIP, ELISA
Conjugate
Unconjugated
Cat no : 14480-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | K-562 cells, human brain tissue, mouse spleen tissue, rat brain tissue |
Positive IP detected in | K-562 cells |
Positive IHC detected in | human renal cell carcinoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
KD/KO | See 4 publications below |
WB | See 14 publications below |
IF | See 8 publications below |
IP | See 2 publications below |
ChIP | See 3 publications below |
Product Information
14480-1-AP targets TFE3 in WB, IHC, IF, IP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | TFE3 fusion protein Ag5863 相同性解析による交差性が予測される生物種 |
Full Name | transcription factor binding to IGHM enhancer 3 |
Calculated molecular weight | 62 kDa |
Observed molecular weight | 70 kDa |
GenBank accession number | BC026027 |
Gene symbol | TFE3 |
Gene ID (NCBI) | 7030 |
RRID | AB_2199587 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
The protein encoded by this gene is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. This protein is also a component of other multisubunit complexes e.g. thyroid hormone receptor-(TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors.
Protocols
Product Specific Protocols | |
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WB protocol for TFE3 antibody 14480-1-AP | Download protocol |
IHC protocol for TFE3 antibody 14480-1-AP | Download protocol |
IP protocol for TFE3 antibody 14480-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Cell Death Differ Lysine methylation of PPP1CA by the methyltransferase SUV39H2 disrupts TFEB-dependent autophagy and promotes intervertebral disc degeneration | ||
Autophagy Oxidation of multiple MiT/TFE transcription factors links oxidative stress to transcriptional control of autophagy and lysosome biogenesis. | ||
Autophagy PRCC-TFE3 fusion-mediated PRKN/parkin-dependent mitophagy promotes cell survival and proliferation in PRCC-TFE3 translocation renal cell carcinoma.
| ||
Cell Discov Targeting macrophage TFEB-14-3-3 epsilon Interface by naringenin inhibits abdominal aortic aneurysm. | ||
Bioorg Med Chem Terpenoids isolated from Chinese liverworts Lepidozia reptans and their anti-inflammatory activity. | ||
Biochim Biophys Acta Gen Subj Anti-cancer effect of marchantin C via inducing lung cancer cellular senescence associated with less secretory phenotype. |