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  • KD/KO Validated

TFE3 Polyclonal antibody

TFE3 Polyclonal Antibody for WB, IP, IHC, ELISA
Cat No. 14480-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, ChIP, ELISA

bHLHe33, RCCP2, TFE3, TFEA, Transcription factor E3

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -


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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター(国内)に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。


保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換/補填対応を承ります。詳細はこちらをご覧ください。


Tested Applications

Positive WB detected inK-562 cells, human brain tissue, mouse spleen tissue, rat brain tissue
Positive IP detected inK-562 cells
Positive IHC detected inhuman renal cell carcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14480-1-AP targets TFE3 in WB, IHC, IF, IP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TFE3 fusion protein Ag5863 相同性解析による交差性が予測される生物種
Full Name transcription factor binding to IGHM enhancer 3
Calculated molecular weight 62 kDa
Observed molecular weight 70 kDa
GenBank accession numberBC026027
Gene Symbol TFE3
Gene ID (NCBI) 7030
RRIDAB_2199587
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP19532
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The protein encoded by this gene is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. This protein is also a component of other multisubunit complexes e.g. thyroid hormone receptor-(TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors.

Protocols

Product Specific Protocols
WB protocol for TFE3 antibody 14480-1-APDownload protocol
IHC protocol for TFE3 antibody 14480-1-APDownload protocol
IP protocol for TFE3 antibody 14480-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Cell Death Differ

Lysine methylation of PPP1CA by the methyltransferase SUV39H2 disrupts TFEB-dependent autophagy and promotes intervertebral disc degeneration

Authors - Huaizhen Liang
humanWB

Autophagy

Oxidation of multiple MiT/TFE transcription factors links oxidative stress to transcriptional control of autophagy and lysosome biogenesis.

Authors - Hongfeng Wang
humanWB,IF,IP

Autophagy

PRCC-TFE3 fusion-mediated PRKN/parkin-dependent mitophagy promotes cell survival and proliferation in PRCC-TFE3 translocation renal cell carcinoma.

Authors - Bo Wang
  • KD Validated
mouseWB

Cell Discov

Targeting macrophage TFEB-14-3-3 epsilon Interface by naringenin inhibits abdominal aortic aneurysm.

Authors - Yiting Jia
humanChIP

J Biol Chem

TFE3-SLC36A1 axis promotes resistance to glucose starvation in kidney cancer cells

Authors - Suli Lv
humanWB,IF

Bioorg Med Chem

Terpenoids isolated from Chinese liverworts Lepidozia reptans and their anti-inflammatory activity.

Authors - Siwen Li
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