Validation Data Gallery
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Tested Applications
| Positive WB detected in | HEK-293 cells, C2C12 cells, pig spleen tissue, HeLa cells, Raji cells, K-562 cells, rat spleen tissue |
| Positive IHC detected in | human malignant melanoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
67319-1-Ig targets TFE3 in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rat, pig samples.
| Tested Reactivity | Human, mouse, rat, pig |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | TFE3 fusion protein Ag6320 相同性解析による交差性が予測される生物種 |
| Full Name | transcription factor binding to IGHM enhancer 3 |
| Calculated molecular weight | 62 kDa |
| Observed molecular weight | 70 kDa |
| GenBank accession number | BC026027 |
| Gene Symbol | TFE3 |
| Gene ID (NCBI) | 7030 |
| RRID | AB_2882579 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P19532 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for TFE3 antibody 67319-1-Ig | Download protocol |
| IHC protocol for TFE3 antibody 67319-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |