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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

TFE3 Monoclonal antibody

TFE3 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat, pig

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1E3D7

Cat no : 67319-1-Ig

Synonyms

bHLHe33, RCCP2, TFE3, TFEA, Transcription factor E3

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TFE3 Monoclonal antibody (67319-1-Ig)

    WB analysis using 67319-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67319-1-Ig (TFE3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of C2C12 cells using TFE3 Monoclonal antibody (67319-1-Ig)

    WB analysis of C2C12 using 67319-1-Ig

    C2C12 cells were subjected to SDS PAGE followed by western blot with 67319-1-Ig (TFE3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using TFE3 Monoclonal antibody (67319-1-Ig)

    WB analysis using 67319-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67319-1-Ig (TFE3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human malignant melanoma tissue using TFE3 Monoclonal antibody (67319-1-Ig)

    IHC staining of human malignant melanoma using 67319-1-Ig

    Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 67319-1-Ig (TFE3 antibody) at dilution of 1:200 (under 40x lens)..

  • Immunohistochemistry (IHC) staining of human malignant melanoma tissue using TFE3 Monoclonal antibody (67319-1-Ig)

    IHC staining of human malignant melanoma using 67319-1-Ig

    Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 67319-1-Ig (TFE3 antibody) at dilution of 1:200 (under 10x lens)..

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Tested Applications

Positive WB detected inHEK-293 cells, C2C12 cells, pig spleen tissue, HeLa cells, Raji cells, K-562 cells, rat spleen tissue
Positive IHC detected inhuman malignant melanoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67319-1-Ig targets TFE3 in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rat, pig samples.

Tested Reactivity Human, mouse, rat, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen TFE3 fusion protein Ag6320 相同性解析による交差性が予測される生物種
Full Name transcription factor binding to IGHM enhancer 3
Calculated molecular weight 62 kDa
Observed molecular weight 70 kDa
GenBank accession numberBC026027
Gene symbol TFE3
Gene ID (NCBI) 7030
RRIDAB_2882579
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for TFE3 antibody 67319-1-IgDownload protocol
IHC protocol for TFE3 antibody 67319-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols