Validation Data Gallery

Filter: AllWBIPIHCIF/ICC

WB analysis using 13372-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 13372-1-AP (TFEB antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of Jurkat using 13372-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 13372-1-AP (TFEB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis using 13372-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 13372-1-AP (TFEB antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
WB analysis of NIH/3T3 using 13372-1-AP
NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 13372-1-AP (TFEB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of rat brain using 13372-1-AP
rat brain tissue were subjected to SDS PAGE followed by western blot with 13372-1-AP (TFEB Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of Jurkat using 13372-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 13372-1-AP (TFEB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 13372-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 13372-1-AP (TFEB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of human placenta using 13372-1-AP
human placenta tissue were subjected to SDS PAGE followed by western blot with 13372-1-AP (TFEB antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
IP experiment of Raji using 13372-1-AP
IP result of anti-TFEB (IP:13372-1-AP, 4ug; Detection:13372-1-AP 1:10000) with Raji cells lysate 2800 ug.
IHC staining of human renal cell carcinoma using 13372-1-AP
Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 13372-1-AP (TFEB antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human renal cell carcinoma using 13372-1-AP
Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 13372-1-AP (TFEB antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HeLa using 13372-1-AP
Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using TFEB antibody (13372-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Featured Product
KD/KO Validated

TFEB Polyclonal antibody

TFEB Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 13372-1-AP

Publications(213)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (3)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA, Wes Immunoassay

Synonyms

Transcription factor EB, TCFEB, Class E basic helix-loop-helix protein 35, bHLHe35, AlphaTFEB

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488 CoraLite®594

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (213)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	A549 cells, Jurkat cells, HeLa cells, human placenta tissue, NIH/3T3 cells, rat brain tissue, HEK-293 cells, Raji cells, C2C12 cells, RAW 264.7 cells, mouse heart tissue, rat heart tissue
Positive IP detected in	Raji cells
Positive IHC detected in	human renal cell carcinoma tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:6000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 27 publications below
WB	See 176 publications below
IHC	See 20 publications below
IF	See 96 publications below
IP	See 6 publications below
CoIP	See 2 publications below

Product Information

13372-1-AP targets TFEB in WB, IHC, IF/ICC, IP, CoIP, ELISA, Wes Immunoassay applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat, monkey, zebrafish, bovine
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	TFEB fusion protein Ag4194 相同性解析による交差性が予測される生物種
Full Name	transcription factor EB
Calculated molecular weight	476 aa, 53 kDa
Observed molecular weight	65-70 kDa
GenBank accession number	BC032448
Gene Symbol	TFEB
Gene ID (NCBI)	7942
RRID	AB_2199611
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P19484
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Transcription factor EB(TFEB) is one of the transcription factors that contain a basic HLH-ZIP domain(for DNA binding ) and HLH and ZIP domains( for loigomerization). TFEB specifically recognizes and binds to E-box sequences, which required dimerization with itself or another MiT/TFE family member. It plays a role in T-cell-dependent antibody responses to activated CD4+ T-cells and thymus-dependent humoral immunity once associated with TFE3. It also activates lysosomal gene by binding the CLEAR-box sequence in the regulatory region. It involves in the signal transduction processes required for normal vascularization of the placenta. This is a rabbit polyclonal antibody raised against the full length of human TFEB. The calculated molecular weight of TFEB is 53 kDa, but modified TFEB is about 65-70 kDa (PMID: 12837690). TFEB mainly present in the cytoplasm (PubMed:23434374). Under aberrant lysosomal storage conditions, it translocates from the cytoplasm to the nucleus (PubMed:23434374). Fisetin increases the levels of TFEB localized in nuclei (PMID: 27112200).

Protocols

Product Specific Protocols
WB protocol for TFEB antibody 13372-1-AP	Download protocol
IHC protocol for TFEB antibody 13372-1-AP	Download protocol
IF protocol for TFEB antibody 13372-1-AP	Download protocol
IP protocol for TFEB antibody 13372-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB	ACS Nano A Self-Assembled α-Synuclein Nanoscavenger for Parkinson's Disease. Authors - Jingyi Liu View Article
mouse	IF	Acta Neuropathol Wolframin is a novel regulator of tau pathology and neurodegeneration. Authors - Shuo Chen View Article
mouse	WB,IF,IP	Mol Cell Itaconate is a lysosomal inducer that promotes antibacterial innate immunity. Authors - Zhenxing Zhang View Article
mouse	WB,IF	J Hazard Mater VPS41-mediated incomplete autophagy aggravates cadmium-induced apoptosis in mouse hepatocytes Authors - Tao Wang View Article
human	WB,IF	Autophagy SMURF1 controls the PPP3/calcineurin complex and TFEB at a regulatory node for lysosomal biogenesis Authors - Qin Xia View Article
human	WB	Cell Death Differ Lysine methylation of PPP1CA by the methyltransferase SUV39H2 disrupts TFEB-dependent autophagy and promotes intervertebral disc degeneration Authors - Huaizhen Liang KD Validated View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 13372-1-AP

WB analysis of Jurkat using 13372-1-AP

WB analysis using 13372-1-AP

WB analysis of NIH/3T3 using 13372-1-AP

WB analysis of rat brain using 13372-1-AP

WB analysis of Jurkat using 13372-1-AP

WB analysis of HeLa using 13372-1-AP

WB analysis of human placenta using 13372-1-AP

IP experiment of Raji using 13372-1-AP

IHC staining of human renal cell carcinoma using 13372-1-AP

IHC staining of human renal cell carcinoma using 13372-1-AP

IF Staining of HeLa using 13372-1-AP

TFEB Polyclonal antibody

TFEB Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

A Self-Assembled α-Synuclein Nanoscavenger for Parkinson's Disease.

Wolframin is a novel regulator of tau pathology and neurodegeneration.

Itaconate is a lysosomal inducer that promotes antibacterial innate immunity.

VPS41-mediated incomplete autophagy aggravates cadmium-induced apoptosis in mouse hepatocytes

SMURF1 controls the PPP3/calcineurin complex and TFEB at a regulatory node for lysosomal biogenesis

Lysine methylation of PPP1CA by the methyltransferase SUV39H2 disrupts TFEB-dependent autophagy and promotes intervertebral disc degeneration