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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse testis tissue using TFEC Polyclonal antibody (13547-1-AP)

    WB analysis of mouse testis using 13547-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 13547-1-AP (TFEC antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of COLO 320 cells using TFEC Polyclonal antibody (13547-1-AP)

    WB analysis of COLO 320 using 13547-1-AP

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 13547-1-AP (TFEC antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse testis tissue using TFEC Polyclonal antibody (13547-1-AP)

    IP experiment of mouse testis using 13547-1-AP

    IP result of anti-TFEC (IP:13547-1-AP, 4ug; Detection:13547-1-AP 1:500) with mouse testis tissue lysate 4000ug.

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TFEC Polyclonal antibody

TFEC Polyclonal Antibody for WB, IP, ELISA
Cat No. 13547-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, ELISA

Synonyms

bHLHe34, hTFEC L, TCFEC, TFE C, TFEC, TFECL, transcription factor EC, Transcription factor EC like

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration:  150 μL, 450 ug/ml
20 μL, 450 ug/ml
150 μL, 450 ug/ml

¥67,000/ 150 μL

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Tested Applications

Positive WB detected inmouse testis tissue, COLO 320 cells
Positive IP detected inmouse testis tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13547-1-AP targets TFEC in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TFEC fusion protein Ag4061 相同性解析による交差性が予測される生物種
Full Name transcription factor EC
Calculated molecular weight 347 aa, 39 kDa
Observed molecular weight35-40 kDa, 65 kDa
GenBank accession numberBC029891
Gene Symbol TFEC
Gene ID (NCBI) 22797
RRIDAB_2199629
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO14948
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The DNA-binding factor TFE3 contains adjacent helix-loop-helix (HLH) and leucine zipper (LZ) domains flanked by an upstream basic region. These protein motifs are frequently observed in other transcription factors and are particularly common to members of the Myc family. TFEC shares a bHLH/LZ structure with TFE3 and a closely related protein microphthalmia-associated transcription factor (MITF), which is critically involved in melanocyte differentiation. The expression of TFEC is mainly in fibroblasts, myoblasts, chondrosarcoma cells and myeloma cells. 50kDa TFEC isoforms are produced by alternative splicing (PMID: 29303964, PMID: 11467950).

Protocols

Product Specific Protocols
WB protocol for TFEC antibody 13547-1-APDownload protocol
IP protocol for TFEC antibody 13547-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols