TFEC Polyclonal antibody
TFEC Polyclonal Antibody for WB, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IP, ELISA
Conjugate
Unconjugated
Cat no : 13547-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse testis tissue, COLO 320 cells |
| Positive IP detected in | mouse testis tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
13547-1-AP targets TFEC in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | TFEC fusion protein Ag4061 相同性解析による交差性が予測される生物種 |
| Full Name | transcription factor EC |
| Calculated molecular weight | 347 aa, 39 kDa |
| Observed molecular weight | 35-40 kDa, 65 kDa |
| GenBank accession number | BC029891 |
| Gene symbol | TFEC |
| Gene ID (NCBI) | 22797 |
| RRID | AB_2199629 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
The DNA-binding factor TFE3 contains adjacent helix-loop-helix (HLH) and leucine zipper (LZ) domains flanked by an upstream basic region. These protein motifs are frequently observed in other transcription factors and are particularly common to members of the Myc family. TFEC shares a bHLH/LZ structure with TFE3 and a closely related protein microphthalmia-associated transcription factor (MITF), which is critically involved in melanocyte differentiation. The expression of TFEC is mainly in fibroblasts, myoblasts, chondrosarcoma cells and myeloma cells. 50kDa TFEC isoforms are produced by alternative splicing (PMID: 29303964, PMID: 11467950).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for TFEC antibody 13547-1-AP | Download protocol |
| IP protocol for TFEC antibody 13547-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Weihao (Verified Customer) (02-04-2022) | Works great for mouse BMDM and human MDM, strong signal.
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