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KD/KO Validated

TFF1 Polyclonal antibody

Name: TFF1 Polyclonal antibody
Brand: Proteintech
SKU: 13734-1-AP

TFF1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 13734-1-AP

Synonyms

Protein pS2, Polypeptide P1.A, PNR-2, pNR 2, HP1.A

Validation Data Gallery

Filter:

AllWBIHCIF/ICC

WB analysis of human stomach using 13734-1-AP
human stomach tissue were subjected to SDS PAGE followed by western blot with 13734-1-AP (Trefoil factor 1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
WB analysis of mouse stomach using 13734-1-AP
mouse stomach tissue were subjected to SDS PAGE followed by western blot with 13734-1-AP (Trefoil factor 1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of human kidney using 13734-1-AP
human kidney tissue were subjected to SDS PAGE followed by western blot with 13734-1-AP (Trefoil factor 1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
IHC staining of human stomach using 13734-1-AP
Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 13734-1-AP (Trefoil factor 1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human stomach using 13734-1-AP
Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 13734-1-AP (Trefoil factor 1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human ovary tumor using 13734-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 13734-1-AP (Trefoil factor 1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human stomach cancer using 13734-1-AP
Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 13734-1-AP (Trefoil factor 1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon cancer using 13734-1-AP
Immunohistochemical analysis of paraffin-embedded Colorectal cancer slide using 13734-1-AP (Trefoil factor 1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon cancer using 13734-1-AP
Immunohistochemical analysis of paraffin-embedded Colorectal cancer slide using 13734-1-AP (Trefoil factor 1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse stomach using 13734-1-AP
Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 13734-1-AP (Trefoil factor 1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse stomach using 13734-1-AP
Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 13734-1-AP (Trefoil factor 1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat stomach using 13734-1-AP
Immunohistochemical analysis of paraffin-embedded rat stomach tissue slide using 13734-1-AP (Trefoil factor 1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat stomach using 13734-1-AP
Immunohistochemical analysis of paraffin-embedded rat stomach tissue slide using 13734-1-AP (Trefoil factor 1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of MCF-7 using 13734-1-AP
Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using Trefoil factor 1 antibody (13734-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Cat No. 13734-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	human stomach tissue, human kidney tissue, mouse stomach tissue
Positive IHC detected in	human stomach tissue, human colon cancer tissue, human ovary tumor tissue, human stomach cancer tissue, mouse stomach tissue, rat stomach tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	MCF-7 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 3 publications below
WB	See 8 publications below
IHC	See 5 publications below
IF	See 1 publications below

Product Information

13734-1-AP targets TFF1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	TFF1 fusion protein Ag4541 相同性解析による交差性が予測される生物種
Full Name	trefoil factor 1
Calculated molecular weight	84 aa, 9 kDa
Observed molecular weight	9-12 kDa
GenBank accession number	BC032811
Gene symbol	TFF1
Gene ID (NCBI)	7031
RRID	AB_2202853
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

TFF1 (Trefoil factor 1, formerly known as pS2) is a small-secreted protein that belongs to the family of trefoil peptides, and characterized by the presence of one to six cysteine-rich P domains. TFF1 expression is frequently lost in gastric carcinoma, probably through mechanism of DNA methylation, and it is therefore considered as a tumor suppressor gene. The function of TFF1 was first controversially considered as an oncogene in breast cancer.

Protocols

Product Specific Protocols
WB protocol for TFF1 antibody 13734-1-AP	Download protocol
IHC protocol for TFF1 antibody 13734-1-AP	Download protocol
IF protocol for TFF1 antibody 13734-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	IF	J Exp Med Single-cell transcriptome analysis reveals differential nutrient absorption functions in human intestine. Authors - Yalong Wang View Article
human	IHC	JCI Insight Resolving the difference between left-sided and right-sided colorectal cancer by single-cell sequencing. Authors - Wei Guo View Article
human	IHC, WB	Bone Trefoil factor-1 upregulation in estrogen-receptor positive breast cancer correlates with an increased risk of bone metastasis. Authors - Chiara Spadazzi KO Validated View Article
human	WB, IHC	Biomed Pharmacother Trefoil factor 1 (TFF1) is a potential prognostic biomarker with functional significance in breast cancers. Authors - Jie Yi KD Validated View Article
human	WB	Aesthet Surg J Cell-Free Fat Extract Prevents Vaginal Atrophy in Ovariectomized Model Via Promoting Proliferation of Vaginal Keratinocyte and Neovascularization. Authors - Bijun Kang View Article
human	IHC	BMC Med Genomics Distinctions in gastric cancer gene expression signatures derived from laser capture microdissection versus histologic macrodissection. Authors - Kim Hark Kyun HK View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

TFF1 Polyclonal antibody

TFF1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Validation Data Gallery

WB analysis of human stomach using 13734-1-AP

WB analysis of mouse stomach using 13734-1-AP

WB analysis of human kidney using 13734-1-AP

IHC staining of human stomach using 13734-1-AP

IHC staining of human stomach using 13734-1-AP

IHC staining of human ovary tumor using 13734-1-AP

IHC staining of human stomach cancer using 13734-1-AP

IHC staining of human colon cancer using 13734-1-AP

IHC staining of human colon cancer using 13734-1-AP

IHC staining of mouse stomach using 13734-1-AP

IHC staining of mouse stomach using 13734-1-AP

IHC staining of rat stomach using 13734-1-AP

IHC staining of rat stomach using 13734-1-AP

IF Staining of MCF-7 using 13734-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Single-cell transcriptome analysis reveals differential nutrient absorption functions in human intestine.

Resolving the difference between left-sided and right-sided colorectal cancer by single-cell sequencing.

Trefoil factor-1 upregulation in estrogen-receptor positive breast cancer correlates with an increased risk of bone metastasis.

Trefoil factor 1 (TFF1) is a potential prognostic biomarker with functional significance in breast cancers.

Cell-Free Fat Extract Prevents Vaginal Atrophy in Ovariectomized Model Via Promoting Proliferation of Vaginal Keratinocyte and Neovascularization.

Distinctions in gastric cancer gene expression signatures derived from laser capture microdissection versus histologic macrodissection.