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TFF2 Polyclonal antibody

TFF2 Polyclonal Antibody for WB, IHC, IF-P, IP, ELISA
Cat No. 13681-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (2)

Applications

WB, IHC, IF-P, IP, ELISA

SML1, trefoil factor 2, Spasmolytic polypeptide, Spasmolysin, SP

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration: 

-/ -


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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター(国内)に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。


保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換/補填対応を承ります。詳細はこちらをご覧ください。


Tested Applications

Positive WB detected inhuman stomach tissue, human urine sample
Positive IP detected inmouse stomach tissue
Positive IHC detected inhuman stomach tissue, human stomach cancer tissue, mouse stomach tissue, mouse small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse stomach tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13681-1-AP targets TFF2 in WB, IHC, IF-P, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, hamster, gerbil
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TFF2 fusion protein Ag4537 相同性解析による交差性が予測される生物種
Full Name trefoil factor 2
Calculated molecular weight 129 aa, 14 kDa
Observed molecular weight 18-20 kDa
GenBank accession numberBC032820
Gene Symbol TFF2
Gene ID (NCBI) 7032
RRIDAB_10598321
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ03403
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The trefoil factor family (TFF), comprises of three polypeptides, TFF1, TFF2 and TFF3 (7-12 kDa), secreted to mucosal surfaces by mucus producing cells, prominently in the gastrointestinal tract. TFF2, also known as spasmolytic polypeptide, is a low-molecular weight protein, expressed in mucous neck cells of the fundus and glands at the base of the antrum in normal human stomach. TFF2 could Inhibit gastrointestinal motility and gastric acid secretion. However, recent studies suggest that TFF2 could also play an important role in the immune system. We got a 18-20 kDa band in western botting maybe due to glycosylatation, and mature TFF2 is a 12 kDa protein (PMID: 10716671).

Protocols

Product Specific Protocols
WB protocol for TFF2 antibody 13681-1-APDownload protocol
IHC protocol for TFF2 antibody 13681-1-APDownload protocol
IF protocol for TFF2 antibody 13681-1-APDownload protocol
IP protocol for TFF2 antibody 13681-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

Nature

A revised airway epithelial hierarchy includes CFTR-expressing ionocytes.

Authors - Daniel T Montoro
mouseIF

Nat Genet

Systematic identification of cell-fate regulatory programs using a single-cell atlas of mouse development.

Authors - Lijiang Fei
mouseIF,IHC

Nat Cell Biol

Lgr5-expressing chief cells drive epithelial regeneration and cancer in the oxyntic stomach.

Authors - Marc Leushacke
human,mouseIHC,IF

Nat Cell Biol

A tumour-resident Lgr5+ stem-cell-like pool drives the establishment and progression of advanced gastric cancers.

Authors - A Fatehullah
humanIF

Gut

Dissecting transcriptional heterogeneity in primary gastric adenocarcinoma by single cell RNA sequencing.

Authors - Min Zhang
mouseIF

Gastroenterology

Induction of gastric cancer by successive oncogenic activation in the corpus.

Authors - Daisuke Douchi
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