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Validation Data Gallery

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  • Western Blot (WB) analysis of rat pancreas tissue using TFF2 Monoclonal antibody (66471-1-Ig)

    WB analysis of rat pancreas using 66471-1-Ig

    rat pancreas tissue were subjected to SDS PAGE followed by western blot with 66471-1-Ig (TFF2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of pig stomach tissue using TFF2 Monoclonal antibody (66471-1-Ig)

    WB analysis of pig stomach using 66471-1-Ig

    pig stomach tissue were subjected to SDS PAGE followed by western blot with 66471-1-Ig (TFF2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of pig pancreas tissue using TFF2 Monoclonal antibody (66471-1-Ig)

    WB analysis of pig pancreas using 66471-1-Ig

    pig pancreas tissue were subjected to SDS PAGE followed by western blot with 66471-1-Ig (TFF2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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TFF2 Monoclonal antibody

TFF2 Monoclonal Antibody for WB, ELISA
Cat No. 66471-1-Ig
Clone No.2D7B1

Host / Isotype

Mouse / IgG2a

Reactivity

human, pig, rat

Applications

WB, ELISA

Synonyms

SML1, SP, Spasmolysin, Spasmolytic polypeptide, TFF2, trefoil factor 2

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inrat pancreas tissue, pig pancreas tissue, pig stomach tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66471-1-Ig targets TFF2 in WB, ELISA applications and shows reactivity with human, pig, rat samples.

Tested Reactivity human, pig, rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen TFF2 fusion protein Ag18765 相同性解析による交差性が予測される生物種
Full Name trefoil factor 2
Calculated molecular weight 129 aa, 14 kDa
Observed molecular weight 16 kDa
GenBank accession numberBC032820
Gene Symbol TFF2
Gene ID (NCBI) 7032
RRIDAB_2881837
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The trefoil factor family (TFF), comprises of three polypeptides, TFF1, TFF2 and TFF3 (7-12 kDa), secreted to mucosal surfaces by mucus producing cells, prominently in the gastrointestinal tract. TFF2, also known as spasmolytic polypeptide, is a low-molecular weight protein, expressed in mucous neck cells of the fundus and glands at the base of the antrum in normal human stomach. TFF2 could Inhibit gastrointestinal motility and gastric acid secretion. However, recent studies suggest that TFF2 could also play an important role in the immune system.

Protocols

Product Specific Protocols
WB protocol for TFF2 antibody 66471-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols