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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TFIP11 Monoclonal antibody (66114-1-Ig)

    WB analysis using 66114-1-Ig (same clone as 66114-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66114-1-Ig (TFIP11 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66114-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using TFIP11 Monoclonal antibody (66114-1-Ig)

    WB analysis using 66114-1-Ig (same clone as 66114-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66114-1-Ig (TFIP11 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control. This data was developed using the same antibody clone with 66114-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using TFIP11 Monoclonal antibody (66114-1-Ig)

    WB analysis using 66114-1-Ig (same clone as 66114-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66114-1-Ig (TFIP11 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66114-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using TFIP11 Monoclonal antibody (66114-1-Ig)

    WB analysis of HeLa using 66114-1-Ig (same clone as 66114-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 66114-1-Ig (TFIP11 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66114-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human testis tissue using TFIP11 Monoclonal antibody (66114-1-Ig)

    WB analysis of human testis using 66114-1-Ig (same clone as 66114-1-PBS)

    human testis tissue were subjected to SDS PAGE followed by western blot with 66114-1-Ig (TFIP11 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66114-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human brain tissue using TFIP11 Monoclonal antibody (66114-1-Ig)

    WB analysis of human brain using 66114-1-Ig (same clone as 66114-1-PBS)

    human brain tissue were subjected to SDS PAGE followed by western blot with 66114-1-Ig (TFIP11 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66114-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using TFIP11 Monoclonal antibody (66114-1-Ig)

    IHC staining of human breast cancer using 66114-1-Ig (same clone as 66114-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66114-1-Ig (TFIP11 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66114-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using TFIP11 Monoclonal antibody (66114-1-Ig)

    IHC staining of human prostate cancer using 66114-1-Ig (same clone as 66114-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66114-1-Ig (TFIP11 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66114-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using TFIP11 Monoclonal antibody (66114-1-Ig)

    IHC staining of human prostate cancer using 66114-1-Ig (same clone as 66114-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66114-1-Ig (TFIP11 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66114-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using TFIP11 Monoclonal antibody (66114-1-Ig)

    IHC staining of human liver cancer using 66114-1-Ig (same clone as 66114-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66114-1-Ig (TFIP11 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66114-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using TFIP11 Monoclonal antibody (66114-1-Ig)

    IHC staining of human liver cancer using 66114-1-Ig (same clone as 66114-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66114-1-Ig (TFIP11 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66114-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using TFIP11 Monoclonal antibody (66114-1-Ig)

    IHC staining of human breast cancer using 66114-1-Ig (same clone as 66114-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66114-1-Ig (TFIP11 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66114-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using TFIP11 Monoclonal antibody (66114-1-Ig)

    IF Staining of HeLa using 66114-1-Ig (same clone as 66114-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using TFIP11 antibody (66114-1-Ig, Clone: 1A1C8 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66114-1-PBS in a different storage buffer formulation.

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TFIP11 Monoclonal antibody, PBS Only

TFIP11 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 66114-1-PBS
Clone No.1A1C8

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

Septin and tuftelin-interacting protein 1, NTR1, HSPC006, hNtr1, bK445C9.6

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66114-1-PBS targets TFIP11 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen TFIP11 fusion protein Ag5821 相同性解析による交差性が予測される生物種
Full Name tuftelin interacting protein 11
Calculated molecular weight 97 kDa
Observed molecular weight 97 kDa
GenBank accession numberBC033080
Gene Symbol TFIP11
Gene ID (NCBI) 24144
RRIDAB_2881513
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9UBB9
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

TFIP11, also named as Tuftelin-interacting protein 11 or Septin and tuftelin-interacting protein 1, is a 837 amino acid protein, which contains one G-patch domain and belongs to the TFP11/STIP family. TFIP11 may localizes in the nucleus and cytoplasm. TFIP11 is involved in pre-mRNA splicing, specifically in spliceosome disassembly during late-stage splicing events. TFIP11 may play a role in the differentiation of ameloblasts and odontoblasts or in the forming of the enamel extracellular matrix.