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Validation Data Gallery

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  • Western Blot (WB) analysis of K-562 cells using TFPT Polyclonal antibody (10097-2-AP)

    WB analysis of K-562 using 10097-2-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 10097-2-AP (TFPT antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HL-60 cells using TFPT Polyclonal antibody (10097-2-AP)

    WB analysis of HL-60 using 10097-2-AP

    HL-60 cells were subjected to SDS PAGE followed by western blot with 10097-2-AP (TFPT antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse liver tissue using TFPT Polyclonal antibody (10097-2-AP)

    WB analysis of mouse liver using 10097-2-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 10097-2-AP (TFPT Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of PC-3 cells using TFPT Polyclonal antibody (10097-2-AP)

    WB analysis of PC-3 using 10097-2-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 10097-2-AP (TFPT antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using TFPT Polyclonal antibody (10097-2-AP)

    IHC staining of mouse liver using 10097-2-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 10097-2-AP (TFPT antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human brain tissue using TFPT Polyclonal antibody (10097-2-AP)

    IHC staining of human brain using 10097-2-AP

    Immunohistochemical analysis of paraffin-embedded human brain slide using 10097-2-AP (TFPT Antibody) at dilution of 1:50.

  • Immunohistochemistry (IHC) staining of human brain tissue using TFPT Polyclonal antibody (10097-2-AP)

    IHC staining of human brain using 10097-2-AP

    Immunohistochemical analysis of paraffin-embedded human brain slide using 10097-2-AP (TFPT Antibody) at dilution of 1:50.

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TFPT Polyclonal antibody

TFPT Polyclonal Antibody for WB, IHC, ELISA
Cat No. 10097-2-AP
Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Synonyms

TCF3 fusion partner, Protein FB1, INO80F, INO80 complex subunit F, FB1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inK-562 cells, HL-60 cells, mouse liver tissue, PC-3 cells
Positive IHC detected inmouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

10097-2-AP targets TFPT in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TFPT fusion protein Ag0131 相同性解析による交差性が予測される生物種
Full Name TCF3 (E2A) fusion partner (in childhood Leukemia)
Calculated molecular weight 29 kDa
Observed molecular weight 29 kDa
GenBank accession numberBC001728
Gene Symbol TFPT
Gene ID (NCBI) 29844
RRIDAB_2240572
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP0C1Z6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TFPT/FB1 is one of three fusion partners of the E2A gene. TFPT/FB1 is expressed at low level in a variety of human tissues, including hemopoietic cell lines from different lineages. The function of the putative TFPT/FB1 protein, however, is currently unknown as database sequence comparisons have failed to reveal strong homology with known proteins. The E2A-TFPT/FB1 fusion appears to be a recurrent feature of pre-B ALLs, suggesting that it might have a role in the development and/or progression of leukemogenesis. The gene encoding TFPT/FB1 is localized on 19q13.4.

Protocols

Product Specific Protocols
WB protocol for TFPT antibody 10097-2-APDownload protocol
IHC protocol for TFPT antibody 10097-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

EMBO J

LncRNA HAND2-AS1 promotes liver cancer stem cell self-renewal via BMP signaling.

Authors - Yanying Wang
View Article
mouseWB

EMBO J

Linc-MYH configures INO80 to regulate muscle stem cell numbers and skeletal muscle hypertrophy.

Authors - Christian Schutt
View Article