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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TGF Beta 1 Recombinant antibody (81746-2-RR)

    WB analysis using 81746-2-RR (same clone as 81746-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 81746-2-RR (TGFB1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81746-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using TGF Beta 1 Recombinant antibody (81746-2-RR)

    WB analysis of HepG2 using 81746-2-RR (same clone as 81746-2-PBS)

    WB result of TGF Beta 1 antibody (81746-2-RR; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TGF Beta 1 transfected HepG2 cells. This data was developed using the same antibody clone with 81746-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using TGF Beta 1 Recombinant antibody (81746-2-RR)

    WB analysis using 81746-2-RR (same clone as 81746-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 81746-2-RR (TGF Beta 1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81746-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using TGF Beta 1 Recombinant antibody (81746-2-RR)

    WB analysis using 81746-2-RR (same clone as 81746-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 81746-2-RR (TGFB1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81746-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse lung tissue using TGF Beta 1 Recombinant antibody (81746-2-RR)

    IHC staining of mouse lung using 81746-2-RR (same clone as 81746-2-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 81746-2-RR (TGF Beta 1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81746-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse lung tissue using TGF Beta 1 Recombinant antibody (81746-2-RR)

    IHC staining of mouse lung using 81746-2-RR (same clone as 81746-2-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 81746-2-RR (TGF Beta 1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81746-2-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using TGF Beta 1 Recombinant antibody (81746-2-RR)

    IF Staining of HEK-293 using 81746-2-RR (same clone as 81746-2-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using TGFB1 antibody (81746-2-RR, Clone: 230544B7 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 81746-2-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HEK-293 cells using TGF Beta 1 Recombinant antibody (81746-2-RR)

    FC experiment of HEK-293 using 81746-2-RR (same clone as 81746-2-PBS)

    1x10^6 HEK-293 cells were intracellularly stained with 0.25 ug TGF Beta 1 Recombinant antibody (81746-2-RR, Clone:230544B7) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 81746-2-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 81746-2-RR

    Affinity and Kinetic Characterization of 81746-2-RR

    Biolayer interferometry (BLl) kinetic assays of 81746-2-RR against Human TGFB1 were performed. The affinity constant is 9.65 nM.

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  • Featured Product
  • KD/KO Validated

TGF Beta 1 Recombinant antibody, PBS Only

TGF Beta 1 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 81746-2-PBS
Clone No.230544B7

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Synonyms

TGFB1, TGFB1,TGF beta 1,TGFbeta,TGF-beta 1, TGF-beta1, 230544B7, Latency-associated peptide

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

81746-2-PBS targets TGF Beta 1 in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen TGF Beta 1 fusion protein Ag13591 相同性解析による交差性が予測される生物種
Full Name transforming growth factor, beta 1
Calculated molecular weight 44 kDa
Observed molecular weight44 kDa
GenBank accession numberBC000125
Gene Symbol TGFB1
Gene ID (NCBI) 7040
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP01137
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

TGF Beta 1 (TGF-β1) is a pleiotropic cytokine and a member of the TGF-β superfamily (including TGF-β1, TGF-β2, and TGF-β3). It is encoded by the TGFB1 gene and acts as a secreted protein involved in controlling cell growth, proliferation, differentiation, and apoptosis. TGF-β1 is produced by various cell types (e.g., platelets, lymphocytes, macrophages, epithelial cells, and inflammatory cells) and is secreted as a precursor requiring proteolytic activation to yield the mature, biologically active peptide. TGF-β1 plays crucial roles in immune regulation, wound healing, fibrosis, and tissue remodeling (including cardiac injury and fibrosis). It also influences cancer progression by promoting epithelial-to-mesenchymal transition (EMT) and metastasis in tumors.