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KD/KO Validated

HIC5 Polyclonal antibody

Name: HIC5 Polyclonal antibody
Brand: Proteintech
SKU: 10565-1-AP

HIC5 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 10565-1-AP

Synonyms

Transforming growth factor beta-1-induced transcript 1 protein, TGFB1I1, Hydrogen peroxide-inducible clone 5 protein, Hic-5, ARA55

Validation Data Gallery

Filter:

AllWBIPIHCIF/ICC

WB analysis using 10565-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10565-1-AP (HIC5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis using 10565-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10565-1-AP (HIC5 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of A431 using 10565-1-AP
A431 cells were subjected to SDS PAGE followed by western blot with 10565-1-AP (HIC5 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of A431 using 10565-1-AP
IP result of anti-HIC5 (IP:10565-1-AP, 4ug; Detection:10565-1-AP 1:500) with A431 cells lysate 1520ug.
IHC staining of human spleen using 10565-1-AP
Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 10565-1-AP (HIC5 antibody at dilution of 1:50 (under 10x lens).
IHC staining of human spleen using 10565-1-AP
Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 10565-1-AP (HIC5 antibody at dilution of 1:50 (under 40x lens).
IF Staining of A431 using 10565-1-AP
Immunofluorescent analysis of A431 cells using 10565-1-AP (HIC5 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).
IF Staining of A549 using 10565-1-AP
Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using HIC5 antibody (10565-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Cat No. 10565-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	HeLa cells, A431 cells, mouse colon tissue, rat colon tissue
Positive IP detected in	A431 cells
Positive IHC detected in	human spleen tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	A431 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:6000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:20-1:200
Immunofluorescence (IF)/ICC	IF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 7 publications below
IHC	See 5 publications below
IF	See 4 publications below

Product Information

10565-1-AP targets HIC5 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	HIC5 fusion protein Ag0868 相同性解析による交差性が予測される生物種
Full Name	transforming growth factor beta 1 induced transcript 1
Calculated molecular weight	48 kDa
Observed molecular weight	48-50 kDa
GenBank accession number	BC001830
Gene symbol	HIC5
Gene ID (NCBI)	7041
RRID	AB_2202045
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for HIC5 antibody 10565-1-AP	Download protocol
IHC protocol for HIC5 antibody 10565-1-AP	Download protocol
IF protocol for HIC5 antibody 10565-1-AP	Download protocol
IP protocol for HIC5 antibody 10565-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse,rat	IF,WB,IHC	Acta Pharmacol Sin GPR97 deficiency ameliorates renal interstitial fibrosis in mouse hypertensive nephropathy Authors - Ji-Chao Wu View Article
human	WB,IF,IHC	Aging (Albany NY) Exosome-mediated Hic-5 regulates proliferation and apoptosis of osteosarcoma via Wnt/β-catenin signal pathway. Authors - Liansheng Sha KD Validated View Article
human	WB	Eur J Pharmacol Vitamin D3 analogue calcipotriol inhibits the profibrotic effects of transforming growth factor- β1 on pancreatic stellate cells Authors - Meifang Zheng View Article
human	WB	J Proteome Res Quantitative proteomics implicates Rictor/mTORC2 in cell adhesion. Authors - Hao Wang View Article
human	IHC,IF	Cell Death Dis HIC-5 in cancer-associated fibroblasts contributes to esophageal squamous cell carcinoma progression. Authors - Xuanling Du View Article
human	IHC	Med Sci Monit Bioinformatics Profiling and Experimental Validation of 4 Differentially-Expressed LIM Genes in the Course of Colorectal-Adenoma-Carcinoma Authors - Ying He View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

HIC5 Polyclonal antibody

HIC5 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Validation Data Gallery

WB analysis using 10565-1-AP

WB analysis using 10565-1-AP

WB analysis of A431 using 10565-1-AP

IP experiment of A431 using 10565-1-AP

IHC staining of human spleen using 10565-1-AP

IHC staining of human spleen using 10565-1-AP

IF Staining of A431 using 10565-1-AP

IF Staining of A549 using 10565-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

GPR97 deficiency ameliorates renal interstitial fibrosis in mouse hypertensive nephropathy

Exosome-mediated Hic-5 regulates proliferation and apoptosis of osteosarcoma via Wnt/β-catenin signal pathway.

Vitamin D3 analogue calcipotriol inhibits the profibrotic effects of transforming growth factor- β1 on pancreatic stellate cells

Quantitative proteomics implicates Rictor/mTORC2 in cell adhesion.

HIC-5 in cancer-associated fibroblasts contributes to esophageal squamous cell carcinoma progression.

Bioinformatics Profiling and Experimental Validation of 4 Differentially-Expressed LIM Genes in the Course of Colorectal-Adenoma-Carcinoma