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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

TGFB2/TGF-beta 2 Recombinant antibody

TGFB2/TGF-beta 2 Recombinant Antibody for IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

230461F5

Cat no : 83167-5-RR

Synonyms

TGFB2-Specific, TGFB2, TGF-beta 2, TGF beta 2, TGF B2

Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of human intrahepatic cholangiocarcinoma tissue using TGFB2/TGF-beta 2 Recombinant antibody (83167-5-RR)

    IHC staining of human intrahepatic cholangiocarcinoma using 83167-5-RR

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 83167-5-RR (TGFB2/TGF-beta 2 antibody) at dilution of 1:250 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human intrahepatic cholangiocarcinoma tissue using TGFB2/TGF-beta 2 Recombinant antibody (83167-5-RR)

    IHC staining of human intrahepatic cholangiocarcinoma using 83167-5-RR

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 83167-5-RR (TGFB2/TGF-beta 2 antibody) at dilution of 1:250 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using TGFB2/TGF-beta 2 Recombinant antibody (83167-5-RR)

    IF Staining of HeLa using 83167-5-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using TGFB2-Specific antibody (83167-5-RR, Clone: 230461F5 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1).

  • Flow cytometry (FC) experiment of A549 cells using TGFB2/TGF-beta 2 Recombinant antibody (83167-5-RR)

    FC experiment of A549 using 83167-5-RR

    1x10^6 A549 cells were intracellularly stained with 0.25 ug Tgfb2-Specific Recombinant Antibody (83167-5-RR, Clone:230461F5) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Flow cytometry (FC) experiment of U-87 MG cells using TGFB2/TGF-beta 2 Recombinant antibody (83167-5-RR)

    FC experiment of U-87 MG using 83167-5-RR

    1x10^6 U-87 MG cells were intracellularly stained with 0.25 ug Tgfb2-Specific Recombinant Antibody (83167-5-RR, Clone:230461F5) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive IHC detected inhuman intrahepatic cholangiocarcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inA549 cells, U-87 MG cells

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:125-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83167-5-RR targets TGFB2/TGF-beta 2 in IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name transforming growth factor, beta 2
Calculated molecular weight 48 kDa
GenBank accession numberNM_003238
Gene symbol TGFB2
Gene ID (NCBI) 7042
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Tranforming growth factor beta-2 (TGFB2), also named G-TSF, BSC-1 cell growth inhibitor, Polyergin and Cetermin, belongs to the TGF-beta family. TGFB2 is a secreted protein and contains a 19aa signal peptides at its N-terminal. Heterodimers with TGFB1 and with TGFB2 have been found in bone. It was proved to have suppressive effects on interleukin-2 dependent T-cell growth.

Protocols

Product Specific Protocols
IHC protocol for TGFB2/TGF-beta 2 antibody 83167-5-RRDownload protocol
IF protocol for TGFB2/TGF-beta 2 antibody 83167-5-RRDownload protocol
FC protocol for TGFB2/TGF-beta 2 antibody 83167-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols