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  • Featured Product
  • KD/KO Validated

TGFBR2 Monoclonal antibody, PBS Only

TGFBR2 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse , Rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

2D5H7

Cat no : 66636-1-PBS

Synonyms

AAT3, FAA3, LDS1B, LDS2B, MFS2, RIIC, TAAD2, TbetaR II, TGF beta receptor type 2, TGF beta receptor type II, TGF beta type II receptor, TGFbeta RII, TGFBR2, TGFR 2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TGFBR2 Monoclonal antibody (66636-1-Ig)

    WB analysis using 66636-1-Ig (same clone as 66636-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66636-1-Ig (TGFBR2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66636-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using TGFBR2 Monoclonal antibody (66636-1-Ig)

    WB analysis using 66636-1-Ig (same clone as 66636-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66636-1-Ig (TGFBR2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 66636-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293T cells using TGFBR2 Monoclonal antibody (66636-1-Ig)

    WB analysis of HEK-293T using 66636-1-Ig (same clone as 66636-1-PBS)

    WB result of TGFBR2 antibody (66636-1-Ig; 1:30000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TGFBR2 transfected HEK-293T cells. This data was developed using the same antibody clone with 66636-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of U2OS cells using TGFBR2 Monoclonal antibody (66636-1-Ig)

    WB analysis of U2OS using 66636-1-Ig (same clone as 66636-1-PBS)

    U2OS cells were subjected to SDS PAGE followed by western blot with 66636-1-Ig (TGFBR2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66636-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A549 cells using TGFBR2 Monoclonal antibody (66636-1-Ig)

    WB analysis of A549 using 66636-1-Ig (same clone as 66636-1-PBS)

    A549 cells were subjected to SDS PAGE followed by western blot with 66636-1-Ig (TGFBR2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66636-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HSC-T6 cells using TGFBR2 Monoclonal antibody (66636-1-Ig)

    WB analysis of HSC-T6 using 66636-1-Ig (same clone as 66636-1-PBS)

    HSC-T6 cells were subjected to SDS PAGE followed by western blot with 66636-1-Ig (TGFBR2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66636-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of NIH/3T3 cells using TGFBR2 Monoclonal antibody (66636-1-Ig)

    WB analysis of NIH/3T3 using 66636-1-Ig (same clone as 66636-1-PBS)

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66636-1-Ig (TGFBR2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66636-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using TGFBR2 Monoclonal antibody (66636-1-Ig)

    IHC staining of human breast cancer using 66636-1-Ig (same clone as 66636-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66636-1-Ig (TGFBR2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66636-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using TGFBR2 Monoclonal antibody (66636-1-Ig)

    IHC staining of human breast cancer using 66636-1-Ig (same clone as 66636-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66636-1-Ig (TGFBR2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66636-1-PBS in a different storage buffer formulation.

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66636-1-PBS targets TGFBR2 in WB, IHC, ELISA applications and shows reactivity with Human, Mouse , Rat samples.

Tested Reactivity Human, Mouse , Rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen TGFBR2 fusion protein Ag25773 相同性解析による交差性が予測される生物種
Full Name transforming growth factor, beta receptor II (70/80kDa)
Calculated molecular weight 65 kDa
Observed molecular weight 65-80 kDa
GenBank accession numberBC040499
Gene symbol TGFBR2
Gene ID (NCBI) 7048
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS only
Storage ConditionsStore at -80°C.

Background Information

TGF beta Receptor II (TGFBR2) is a member of the transforming growth factor-β (TGF-β) superfamily, which are critical regulators of cell proliferation and differentiation, developmental patterning and morphogenesis, and disease pathogenesis (PMID: 10974075). TGFBR2 result in both SMAD4-dependent constraint of proliferation and SMAD4-independent activation of apoptosis (PMID: 29396446). It has some isoforms with the molecular weight of 70-80 kDa and ~90 kDa after glycosylated.