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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

TGIF2 Monoclonal antibody

TGIF2 Monoclonal Antibody for WB, FC (Intra), ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

2D6B6

Cat no : 67576-1-Ig

Synonyms

TGFB induced factor homeobox 2, TGFB induced factor 2, Homeobox protein TGIF2, 5'-TG-3'-interacting factor 2, 5 TG 3 interacting factor 2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TGIF2 Monoclonal antibody (67576-1-Ig)

    WB analysis using 67576-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67576-1-Ig (TGIF2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of COLO 320 cells using TGIF2 Monoclonal antibody (67576-1-Ig)

    WB analysis of COLO 320 using 67576-1-Ig

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 67576-1-Ig (TGIF2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of THP-1 cells using TGIF2 Monoclonal antibody (67576-1-Ig)

    WB analysis of THP-1 using 67576-1-Ig

    THP-1 cells were subjected to SDS PAGE followed by western blot with 67576-1-Ig (TGIF2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Flow cytometry (FC) experiment of HepG2 cells using TGIF2 Monoclonal antibody (67576-1-Ig)

    FC experiment of HepG2 using 67576-1-Ig

    1x10^6 HepG2 cells were intracellularly stained with 0.4 ug TGIF2 Monoclonal antibody (67576-1-Ig, Clone:2D6B6) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.4 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Tested Applications

Positive WB detected inHepG2 cells, THP-1 cells, COLO 320 cells, Ramos cells, Daudi cells, K-562 cells, Jurkat cells, HL-60 cells, MOLT-4 cells
Positive FC (Intra) detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

67576-1-Ig targets TGIF2 in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen TGIF2 fusion protein Ag30083 相同性解析による交差性が予測される生物種
Full Name TGFB-induced factor homeobox 2
Calculated molecular weight 237 aa, 26 kDa
Observed molecular weight 30 kDa
GenBank accession numberBC012816
Gene symbol TGIF2
Gene ID (NCBI) 60436
RRIDAB_2882789
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TGIF2, is a member of a subfamily of transcription factor with a TALE(3-amino acid loop extension), an atypical homeodomain. It also contains a proline-rich region, which is a putative SH3 domain-binding site and suggested involve in transcriptional regulation. It is highly expressed in the heart, kidney, and testis, but low in the brain and prostate. Through binding directly to the 5'-CTGTCAA-3' DNA sequence or by interacting with TGF-beta activated SMAD proteins, TGIF2 acts as a transcriptional repressor. The calculated molecular weight of TGIF2 is 26 kDa, but the phosphorylated TGIF2 is about 30-35 kDa protein.

Protocols

Product Specific Protocols
WB protocol for TGIF2 antibody 67576-1-IgDownload protocol
FC protocol for TGIF2 antibody 67576-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Bosn J Basic Med Sci

Circ-RNF13, as an oncogene, regulates malignant progression of HBV-associated hepatocellular carcinoma cells and HBV infection through ceRNA pathway of circ-RNF13/miR-424-5p/TGIF2.

Authors - Yan Chen
View Article