TGM1 Polyclonal antibody
TGM1 Polyclonal Antibody for WB, IHC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse and More (1)
Applications
WB, IHC, IF, IP, ELISA
Conjugate
Unconjugated
Cat no : 12912-3-AP
Synonyms
Validation Data Gallery
Filter:
Tested Applications
| Positive WB detected in | human saliva, mouse kidney tissue |
| Positive IP detected in | mouse kidney tissue |
| Positive IHC detected in | human cervical cancer tissue, human bowen disease, human brown disease, human cervix tissue, human skin tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 16 publications below |
| IHC | See 5 publications below |
| IF | See 11 publications below |
| ELISA | See 1 publications below |
Product Information
12912-3-AP targets TGM1 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | TGM1 fusion protein Ag3905 相同性解析による交差性が予測される生物種 |
| Full Name | transglutaminase 1 (K polypeptide epidermal type I, protein-glutamine-gamma-glutamyltransferase) |
| Calculated molecular weight | 817 aa, 90 kDa |
| Observed molecular weight | 90 kDa |
| GenBank accession number | BC034699 |
| Gene symbol | TGM1 |
| Gene ID (NCBI) | 7051 |
| RRID | AB_2271748 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Transglutaminase 1 is an epidermal enzyme that is expressed during the terminal differentiation of keratinized squamous epithelium to form cornified cell envelopes in differentiated keratinocytes. Mutation of TGM1 has been linked to various epidermal diseases. This antibody recognizes the endogenous 90 kDa TGM1 proteins.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for TGM1 antibody 12912-3-AP | Download protocol |
| IHC protocol for TGM1 antibody 12912-3-AP | Download protocol |
| IP protocol for TGM1 antibody 12912-3-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Control Release Superior COL7A1 and TGM1 gene expression in difficult-to-transfect skin cell mediated by highly branched poly(β-amino esters) through stepwise fractionation | ||
J Invest Dermatol Investigation of Omega-3 Polyunsaturated Fatty Acid Biological Activity in a Tissue-Engineered Skin Model Involving Psoriatic Cells. | ||
J Invest Dermatol Cathelicidin LL-37 Activates Human Keratinocyte Autophagy through the P2X₇, Mechanistic Target of Rapamycin, and MAPK Pathways | ||
Int J Mol Sci Docosahexaenoic Acid Modulates Paracellular Absorption of Testosterone and Claudin-1 Expression in a Tissue-Engineered Skin Model. | ||
Cells Increased Osteoclastogenesis in Absence of TG2 Is Reversed by Transglutaminase Inhibition-Evidence for the Role for TG1 in Osteoclast Formation |
