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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

TGM5 Polyclonal antibody

TGM5 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 25071-1-AP

Synonyms

TG(X), TGase 5, TGase X, TGM5, TGM6, TGMX, TGX, transglutaminase 5, Transglutaminase X

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse skin tissue using TGM5 Polyclonal antibody (25071-1-AP)

    WB analysis of mouse skin using 25071-1-AP

    mouse skin tissue were subjected to SDS PAGE followed by western blot with 25071-1-AP (TGM5 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A431 cells using TGM5 Polyclonal antibody (25071-1-AP)

    WB analysis of A431 using 25071-1-AP

    A431 cells were subjected to SDS PAGE followed by western blot with 25071-1-AP (TGM5 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse skin tissue using TGM5 Polyclonal antibody (25071-1-AP)

    IP experiment of mouse skin using 25071-1-AP

    IP result of anti-TGM5 (IP:25071-1-AP, 4ug; Detection:25071-1-AP 1:500) with mouse skin tissue lysate 4000ug.

  • Immunohistochemistry (IHC) staining of human skin tissue using TGM5 Polyclonal antibody (25071-1-AP)

    IHC staining of human skin using 25071-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 25071-1-AP (TGM5 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human skin tissue using TGM5 Polyclonal antibody (25071-1-AP)

    IHC staining of human skin using 25071-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 25071-1-AP (TGM5 Antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

Positive WB detected inmouse skin tissue, A431 cells
Positive IP detected inmouse skin tissue
Positive IHC detected inhuman skin tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 25071-1-AP is TGM5 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TGM5 fusion protein Ag18492 相同性解析による交差性が予測される生物種
Full Name transglutaminase 5
Calculated molecular weight 720 aa, 81 kDa
Observed molecular weight 84 kDa
GenBank accession numberBC122859
Gene symbol TGM5
Gene ID (NCBI) 9333
RRIDAB_2879883
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for TGM5 antibody 25071-1-APDownload protocol
IHC protocol for TGM5 antibody 25071-1-APDownload protocol
IP protocol for TGM5 antibody 25071-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols