Validation Data Gallery

Filter: AllWBIPIHCIF-PIF/ICCIF

WB analysis using 13573-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 13573-1-AP (TGN46 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 13573-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 13573-1-AP (TGN46 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
IP experiment of HeLa using 13573-1-AP
IP result of anti-TGN46 (IP:13573-1-AP, 3ug; Detection:13573-1-AP 1:300) with HeLa cells lysate 2400ug.
IP experiment of HeLa using 13573-1-AP
IP result of anti-TGN46 (IP:13573-1-AP, 3ug; Detection:13573-1-AP 1:300) with HeLa cells lysate 2400ug.
IHC staining of human stomach cancer using 13573-1-AP
Immunohistochemical analysis of paraffin-embedded stomach cancer slide using 13573-1-AP (TGN46 antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of human cerebellum using 13573-1-AP
Immunofluorescent analysis of (4% PFA) fixed human cerebellum tissue using 13573-1-AP (TGN46 antibody), at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of HeLa using 13573-1-AP
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using TGN46 antibody (13573-1-AP) at dilution of 1:2000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).
IF Staining of A549 using 13573-1-AP
Immunofluorescent analysis of (4% PFA) fixed A549 cells using TGN46 antibody (13573-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of HeLa using 13573-1-AP
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 13573-1-AP (TGN46 antibody), at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL555-phalloidin stains F-actin (red).
IF Staining of Fibroblast (primary cells) using 13573-1-AP
IF result of TGN46 antibody (1:50) with Human Fibroblast (primary cells) By Dr. Neeraj Tiwar, Rothman Lab, Yale School of Medicine. Fixed with 4% PFA 10 min.

TGN46 Polyclonal antibody

TGN46 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, IP, ELISA

Cat No. 13573-1-AP

Publications(32)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (4)

Applications

WB, IHC, IF/ICC, IF-P, IP, ELISA

Synonyms

TGOLN2, TGN48, TGN38 homolog, TGN38, hTGN51

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488 CoraLite®594

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (32)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	A549 cells, HeLa cells, HepG2 cells
Positive IP detected in	HeLa cells
Positive IHC detected in	human stomach cancer **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF-P detected in	human cerebellum tissue
Positive IF/ICC detected in	HeLa cells, A549 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:8000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:400-1:1600
Immunofluorescence (IF)-P	IF-P : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 6 publications below
IF	See 26 publications below

Product Information

13573-1-AP targets TGN46 in WB, IHC, IF/ICC, IF-P, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Cited Reactivity	human, mouse, pig, monkey, hamster
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	TGN46 fusion protein Ag4470 相同性解析による交差性が予測される生物種
Full Name	trans-golgi network protein 2
Calculated molecular weight	447 aa, 47 kDa
Observed molecular weight	90-100 kDa
GenBank accession number	BC028219
Gene Symbol	TGN46
Gene ID (NCBI)	10618
RRID	AB_10597396
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	O43493
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

TGN46 (TGOLN2), the human homolog of rat Tgn38, is a transmembrane glycoprotein predominantly localized to the TGN (trans-Golgi network). TGN is a major secretory pathway sorting station for proteins and lipids. TGN46 may be involved in regulating membrane traffic to and from TGN. Alternatively, spliced transcript variants encode different TGN46 isoforms. TGN46 has an apparent molecular mass of 100-150 kDa, suggesting extensive O- and N-glycosylations.

Protocols

Product Specific Protocols
WB protocol for TGN46 antibody 13573-1-AP	Download protocol
IHC protocol for TGN46 antibody 13573-1-AP	Download protocol
IF protocol for TGN46 antibody 13573-1-AP	Download protocol
IP protocol for TGN46 antibody 13573-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
		ACS Cent Sci Biowire Model of Interstitial and Focal Cardiac Fibrosis. Authors - Erika Yan Wang View Article
human	IF	Autophagy Live imaging of intra-lysosome pH in cell lines and primary neuronal culture using a novel genetically encoded biosensor. Authors - Amy H Ponsford View Article
human	IF	Cell Death Differ Oleate-induced aggregation of LC3 at the trans-Golgi network is linked to a protein trafficking blockade. Authors - Giulia Cerrato View Article
human	WB	Autophagy Bunyavirus SFTSV exploits autophagic flux for viral assembly and egress Authors - Jia-min Yan View Article
human	IF	EMBO Rep A conserved ion channel function of STING mediates noncanonical autophagy and cell death Authors - Jinrui Xun View Article
human	IF	PLoS Pathog Host RAB11FIP5 protein inhibits the release of Kaposi's sarcoma-associated herpesvirus particles by promoting lysosomal degradation of ORF45. Authors - Xiaoqin Wei View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 13573-1-AP

WB analysis of HeLa using 13573-1-AP

IP experiment of HeLa using 13573-1-AP

IP experiment of HeLa using 13573-1-AP

IHC staining of human stomach cancer using 13573-1-AP

IF Staining of human cerebellum using 13573-1-AP

IF Staining of HeLa using 13573-1-AP

IF Staining of A549 using 13573-1-AP

IF Staining of HeLa using 13573-1-AP

IF Staining of Fibroblast (primary cells) using 13573-1-AP

TGN46 Polyclonal antibody

TGN46 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Biowire Model of Interstitial and Focal Cardiac Fibrosis.

Live imaging of intra-lysosome pH in cell lines and primary neuronal culture using a novel genetically encoded biosensor.

Oleate-induced aggregation of LC3 at the trans-Golgi network is linked to a protein trafficking blockade.

Bunyavirus SFTSV exploits autophagic flux for viral assembly and egress

A conserved ion channel function of STING mediates noncanonical autophagy and cell death

Host RAB11FIP5 protein inhibits the release of Kaposi's sarcoma-associated herpesvirus particles by promoting lysosomal degradation of ORF45.