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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

TGS1 Polyclonal antibody

TGS1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 13034-1-AP

Synonyms

Hepatocellular carcinoma-associated antigen 137, HCA137, EC:2.1.1.-, CLL-associated antigen KW-2, CLL associated antigen KW 2

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse testis tissue using TGS1 Polyclonal antibody (13034-1-AP)

    WB analysis of mouse testis using 13034-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 13034-1-AP (TGS1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using TGS1 Polyclonal antibody (13034-1-AP)

    IHC staining of human ovary cancer using 13034-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 13034-1-AP (TGS1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inmouse testis tissue
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13034-1-AP targets TGS1 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TGS1 fusion protein Ag3683 相同性解析による交差性が予測される生物種
Full Name trimethylguanosine synthase homolog (S. cerevisiae)
Calculated molecular weight 852 aa, 90 kDa
Observed molecular weight 90 kDa
GenBank accession numberBC011999
Gene symbol TGS1
Gene ID (NCBI) 96764
RRIDAB_2201380
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for TGS1 antibody 13034-1-APDownload protocol
IHC protocol for TGS1 antibody 13034-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols