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KD/KO Validated

THAP1 Polyclonal antibody

Name: THAP1 Polyclonal antibody
Brand: Proteintech
SKU: 12584-1-AP

THAP1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ChIP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 12584-1-AP

Synonyms

THAP1

Validation Data Gallery

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AllWBIHC

WB analysis of HepG2 using 12584-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 12584-1-AP (THAP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of A549 using 12584-1-AP
WB result of THAP1 antibody (12584-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-THAP1 transfected A549 cells.
WB analysis of human brain using 12584-1-AP
human brain tissue were subjected to SDS PAGE followed by western blot with 12584-1-AP (THAP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 12584-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 12584-1-AP (THAP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 12584-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 12584-1-AP (THAP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 12584-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 12584-1-AP (THAP1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of human heart using 12584-1-AP
human heart tissue were subjected to SDS PAGE followed by western blot with 12584-1-AP (THAP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 12584-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 12584-1-AP (THAP1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of mouse heart using 12584-1-AP
mouse heart tissue were subjected to SDS PAGE followed by western blot with 12584-1-AP (THAP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 12584-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 12584-1-AP (THAP1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 12584-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 12584-1-AP (THAP1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of A375 using 12584-1-AP
A375 cells were subjected to SDS PAGE followed by western blot with 12584-1-AP (THAP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of SGC-7901 using 12584-1-AP
SGC-7901 cells were subjected to SDS PAGE followed by western blot with 12584-1-AP (THAP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 12584-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 12584-1-AP (THAP1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
IHC staining of human colon cancer using 12584-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer using 12584-1-AP (THAP1 antibody) at dilution of 1:50 (under 40x lens).

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Cat No. 12584-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	HepG2 cells, human brain tissue, HEK-293 cells, HeLa cells, human heart tissue, A375 cells, SGC-7901 cells, mouse brain tissue, mouse heart tissue
Positive IHC detected in	human colon cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunohistochemistry (IHC)	IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 5 publications below
WB	See 11 publications below
IHC	See 1 publications below
ChIP	See 1 publications below

Product Information

12584-1-AP targets THAP1 in WB, IHC, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	THAP1 fusion protein Ag3285 相同性解析による交差性が予測される生物種
Full Name	THAP domain containing, apoptosis associated protein 1
Calculated molecular weight	213 aa, 25 kDa
Observed molecular weight	25-30 kDa
GenBank accession number	BC021721
Gene symbol	THAP1
Gene ID (NCBI)	55145
RRID	AB_2201672
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

THAP1 belongs to the THAP1 family. It is a DNA-binding transcription regulator that regulates endothelial cell proliferation and G1/S cell-cycle progression. THAP1 may also have pro-apoptopic activity by potentiating both serum-withdrawal and TNF-induced apoptosis. Mutations in THAP1 have been associated with dystonia 6. THAP1 encodes a transcription factor with mostly unknown targets. It regulates the expression of DYT1 (TOR1A), another dystonia-causing gene. After characterization of the TOR1A promoter, THAP1 binds to the core promoter of TOR1A. Wild type THAP1 represses the expression of TOR1A, whereas dystonia 6-associated mutant THAP1 results in decreased repression of TOR1A. Catalog#12584-1-AP is a rabbit polyclonal antibody raised against full-length of huamn THAP1.

Protocols

Product Specific Protocols
WB protocol for THAP1 antibody 12584-1-AP	Download protocol
IHC protocol for THAP1 antibody 12584-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Nat Genet Mutations in the histone methyltransferase gene KMT2B cause complex early-onset dystonia. Authors - Esther Meyer View Article
mouse	WB,ChIP	Mol Cell The dystonia gene THAP1 controls DNA double-strand break repair choice. Authors - Kenta Shinoda KO Validated View Article
human	WB	Brain DYT6 mutated THAP1 is a cell type dependent regulator of the SP1 family. Authors - Fubo Cheng KO Validated View Article
mouse	WB	Ann Neurol Direct interaction between causative genes of DYT1 and DYT6 primary dystonia. Authors - Gavarini Sophie S View Article
human	WB	Ann Neurol The dystonia gene DYT1 is repressed by the transcription factor THAP1 (DYT6). Authors - Kaiser Frank J FJ View Article
mouse	WB	Dev Cell The DYT6 Dystonia Protein THAP1 Regulates Myelination within the Oligodendrocyte Lineage. Authors - Dhananjay Yellajoshyula View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

THAP1 Polyclonal antibody

THAP1 Polyclonal Antibody for WB, IHC, ELISA

Validation Data Gallery

WB analysis of HepG2 using 12584-1-AP

WB analysis of A549 using 12584-1-AP

WB analysis of human brain using 12584-1-AP

WB analysis of HEK-293 using 12584-1-AP

WB analysis of HeLa using 12584-1-AP

WB analysis of HeLa using 12584-1-AP

WB analysis of human heart using 12584-1-AP

WB analysis of mouse brain using 12584-1-AP

WB analysis of mouse heart using 12584-1-AP

WB analysis of HeLa using 12584-1-AP

WB analysis of HEK-293 using 12584-1-AP

WB analysis of A375 using 12584-1-AP

WB analysis of SGC-7901 using 12584-1-AP

WB analysis of HEK-293 using 12584-1-AP

IHC staining of human colon cancer using 12584-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Mutations in the histone methyltransferase gene KMT2B cause complex early-onset dystonia.

The dystonia gene THAP1 controls DNA double-strand break repair choice.

DYT6 mutated THAP1 is a cell type dependent regulator of the SP1 family.

Direct interaction between causative genes of DYT1 and DYT6 primary dystonia.

The dystonia gene DYT1 is repressed by the transcription factor THAP1 (DYT6).

The DYT6 Dystonia Protein THAP1 Regulates Myelination within the Oligodendrocyte Lineage.