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Species Reactivity

Species Reactivity

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  • Featured Product
  • KD/KO Validated

THAP11 Polyclonal antibody

THAP11 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 23030-1-AP

Synonyms

THAP domain-containing protein 11, RONIN, HRIHFB2206, CTG B45d, CTG B43a

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using THAP11 Polyclonal antibody (23030-1-AP)

    WB analysis using 23030-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 23030-1-AP (THAP11 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using THAP11 Polyclonal antibody (23030-1-AP)

    IHC staining of human colon cancer using 23030-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 23030-1-AP (THAP11 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using THAP11 Polyclonal antibody (23030-1-AP)

    IHC staining of human colon cancer using 23030-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 23030-1-AP (THAP11 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inHeLa cells, Jurkat cells, C2C12 cells, NIH/3T3 cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

23030-1-AP targets THAP11 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen THAP11 fusion protein Ag19342 相同性解析による交差性が予測される生物種
Full Name THAP domain containing 11
Calculated molecular weight 314 aa, 34 kDa
Observed molecular weight 40-50 kDa
GenBank accession numberBC012182
Gene symbol THAP11
Gene ID (NCBI) 57215
RRIDAB_2879200
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for THAP11 antibody 23030-1-APDownload protocol
IHC protocol for THAP11 antibody 23030-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Biomed Res Int

THAP11 Functions as a Tumor Suppressor in Gastric Cancer through Regulating c-Myc Signaling Pathways.

Authors - Jing Zhang
  • KD Validated
View Article
mouseWB,IF

iScience

Seizure enhances SUMOylation and zinc-finger transcriptional repression in neuronal nuclei

Authors - Hui Rong Soon
View Article