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Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using THAP2 Polyclonal antibody (20853-1-AP)

    WB analysis of HEK-293 using 20853-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 20853-1-AP (THAP2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse testis tissue using THAP2 Polyclonal antibody (20853-1-AP)

    WB analysis of mouse testis using 20853-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 20853-1-AP (THAP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using THAP2 Polyclonal antibody (20853-1-AP)

    IF Staining of HeLa using 20853-1-AP

    Immunofluorescent analysis of Hela cells, using THAP2 antibody 20853-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using THAP2 Polyclonal antibody (20853-1-AP)

    IF Staining of HepG2 using 20853-1-AP

    Immunofluorescent analysis of HepG2 cells, using THAP2 antibody 20853-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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THAP2 Polyclonal antibody

THAP2 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 20853-1-AP
Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IF/ICC, ELISA

Synonyms

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHEK-293 cells, mouse testis tissue
Positive IF/ICC detected inHela cells, HepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

20853-1-AP targets THAP2 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen THAP2 fusion protein Ag14775 相同性解析による交差性が予測される生物種
Full Name THAP domain containing, apoptosis associated protein 2
Calculated molecular weight 228 aa, 26 kDa
Observed molecular weight 26-30 kDa
GenBank accession numberBC008358
Gene Symbol THAP2
Gene ID (NCBI) 83591
RRIDAB_10732806
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9H0W7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

THAP2, also named as THAP domain-containing protein 2, is a 228 amino acid protein, which contains one THAP-type zinc finger. Members of the THAP (thanatos-associated protein) family of proteins contain a well conserved DNA-binding domain known as the THAP-type zinc finger motif. Proteins containing the THAP-type zinc finger motif are commonly involved in transcriptional regulation, cell-cycle control, apoptosis and chromatin modification

Protocols

Product Specific Protocols
WB protocol for THAP2 antibody 20853-1-APDownload protocol
IF protocol for THAP2 antibody 20853-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB

Cancer Cell Int

The involvement of miR-100 in bladder urothelial carcinogenesis changing the expression levels of mRNA and proteins of genes related to cell proliferation, survival, apoptosis and chromosomal stability.

Authors - Denis R Morais
View Article
humanWB

Clinics (Sao Paulo)

MicroRNA 100: a context dependent miRNA in prostate cancer.

Authors - Leite Katia R M KR
View Article