• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CD141/Thrombomodulin Monoclonal antibody, PBS Only

CD141/Thrombomodulin Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

5A4C5

Cat no : 67831-1-PBS

Synonyms

THBD, Thrombomodulin, THRM, Fetomodulin, CD141

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using CD141/Thrombomodulin Monoclonal antibody (67831-1-Ig)

    WB analysis using 67831-1-Ig (same clone as 67831-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67831-1-Ig (Thrombomodulin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control. This data was developed using the same antibody clone with 67831-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human placenta tissue using CD141/Thrombomodulin Monoclonal antibody (67831-1-Ig)

    WB analysis of human placenta using 67831-1-Ig (same clone as 67831-1-PBS)

    human placenta tissue were subjected to SDS PAGE followed by western blot with 67831-1-Ig (THBD antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67831-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using CD141/Thrombomodulin Monoclonal antibody (67831-1-Ig)

    IHC staining of human lung cancer using 67831-1-Ig (same clone as 67831-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67831-1-Ig (Thrombomodulin antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67831-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using CD141/Thrombomodulin Monoclonal antibody (67831-1-Ig)

    IHC staining of human lung cancer using 67831-1-Ig (same clone as 67831-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67831-1-Ig (Thrombomodulin antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67831-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using CD141/Thrombomodulin Monoclonal antibody (67831-1-Ig)

    IHC staining of human placenta using 67831-1-Ig (same clone as 67831-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 67831-1-Ig (THBD antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67831-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using CD141/Thrombomodulin Monoclonal antibody (67831-1-Ig)

    IHC staining of human placenta using 67831-1-Ig (same clone as 67831-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 67831-1-Ig (THBD antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67831-1-PBS in a different storage buffer formulation.

☆大好評トライアルサイズ ¥20,000～！ >>> 一次抗体特別価格キャンペーン開催中！

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67831-1-PBS targets CD141/Thrombomodulin in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen CD141/Thrombomodulin fusion protein Ag5723 相同性解析による交差性が予測される生物種
Full Name thrombomodulin
Calculated molecular weight 60 kDa
Observed molecular weight105 kDa
GenBank accession numberBC035602
Gene symbol THBD
Gene ID (NCBI) 7056
RRIDAB_2918593
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Thrombomodulin, also known as THBD and CD141, is an endothelial cell surface glycoprotein that forms a 1:1 complex with the coagulation factor thrombin and plays an important role as a natural anticoagulant. Thrombomodulin serves to convert thrombin from a procoagulant protein into the activator for protein C. Once converted to activated protein C (APC), this protein serves as a major anticoagulant in blood (PMID: 2827310). Thrombomodulin is also located in other cells (keratinocytes, osteoblasts, macrophages,...) where it might be involved in cell differentiation or in inflammation (PMID: 9814688). In humans, thrombomodulin is encoded by the THBD gene. Mutations in this gene are a cause of thromboembolic disease, also known as inherited thrombophilia. Thrombomodulin is glycosylated and has an apparent molecular weight of 75 to 110 kDa (PMID: 1650405; 2827310).