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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CD141/Thrombomodulin Monoclonal antibody, PBS Only

CD141/Thrombomodulin Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

5A4C5

Cat no : 67831-1-PBS

Synonyms

THBD, Thrombomodulin, THRM, Fetomodulin, CD141

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CD141/Thrombomodulin Monoclonal antibody (67831-1-Ig)

    WB analysis using 67831-1-Ig (same clone as 67831-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67831-1-Ig (Thrombomodulin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control. This data was developed using the same antibody clone with 67831-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human placenta tissue using CD141/Thrombomodulin Monoclonal antibody (67831-1-Ig)

    WB analysis of human placenta using 67831-1-Ig (same clone as 67831-1-PBS)

    human placenta tissue were subjected to SDS PAGE followed by western blot with 67831-1-Ig (THBD antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67831-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using CD141/Thrombomodulin Monoclonal antibody (67831-1-Ig)

    IHC staining of human lung cancer using 67831-1-Ig (same clone as 67831-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67831-1-Ig (Thrombomodulin antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67831-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using CD141/Thrombomodulin Monoclonal antibody (67831-1-Ig)

    IHC staining of human lung cancer using 67831-1-Ig (same clone as 67831-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67831-1-Ig (Thrombomodulin antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67831-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using CD141/Thrombomodulin Monoclonal antibody (67831-1-Ig)

    IHC staining of human placenta using 67831-1-Ig (same clone as 67831-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 67831-1-Ig (THBD antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67831-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using CD141/Thrombomodulin Monoclonal antibody (67831-1-Ig)

    IHC staining of human placenta using 67831-1-Ig (same clone as 67831-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 67831-1-Ig (THBD antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67831-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67831-1-PBS targets CD141/Thrombomodulin in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen CD141/Thrombomodulin fusion protein Ag5723 相同性解析による交差性が予測される生物種
Full Name thrombomodulin
Calculated molecular weight 60 kDa
Observed molecular weight105 kDa
GenBank accession numberBC035602
Gene symbol THBD
Gene ID (NCBI) 7056
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Thrombomodulin, also known as THBD and CD141, is an endothelial cell surface glycoprotein that forms a 1:1 complex with the coagulation factor thrombin and plays an important role as a natural anticoagulant. Thrombomodulin serves to convert thrombin from a procoagulant protein into the activator for protein C. Once converted to activated protein C (APC), this protein serves as a major anticoagulant in blood (PMID: 2827310). Thrombomodulin is also located in other cells (keratinocytes, osteoblasts, macrophages,...) where it might be involved in cell differentiation or in inflammation (PMID: 9814688). In humans, thrombomodulin is encoded by the THBD gene. Mutations in this gene are a cause of thromboembolic disease, also known as inherited thrombophilia. Thrombomodulin is glycosylated and has an apparent molecular weight of 75 to 110 kDa (PMID: 1650405; 2827310).