Validation Data Gallery
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Tested Applications
| Positive IHC detected in | human liver tissue, human colon tissue, mouse kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 3 publications below |
| IF | See 2 publications below |
Product Information
19727-1-AP targets Thrombospondin 3-Specific in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | thrombospondin 3 |
| Calculated molecular weight | 104 kDa |
| GenBank accession number | NM_007112 |
| Gene Symbol | Thrombospondin 3 |
| Gene ID (NCBI) | 7059 |
| RRID | AB_10695895 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P49746 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
THBS3, also named as TSP3, belongs to the thrombospondin family. THBS3 is an adhesive glycoprotein that mediates cell-to-cell and cell-to-matrix interactions. THBS3 can bind to fibrinogen, fibronectin, laminin and type V collagen. The antibody is specific to THBS3.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for Thrombospondin 3-Specific antibody 19727-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Front Cell Dev Biol Time-Resolved Extracellular Matrix Atlas of the Developing Human Skin Dermis. | ||
Mol Cell Biochem Microrna363-5p targets thrombospondin3 to regulate pathological cardiac remodeling | ||
Nat Commun Thrombospondin-3 augments injury-induced cardiomyopathy by intracellular integrin inhibition and sarcolemmal instability.
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