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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

THEM2 Polyclonal antibody

THEM2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 16782-1-AP

Synonyms

EC:3.1.2.2, EC:3.1.2.-, Acyl-coenzyme A thioesterase 13, N-terminally processed, Acyl-coenzyme A thioesterase 13, Acyl-CoA thioesterase 13

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using THEM2 Polyclonal antibody (16782-1-AP)

    WB analysis using 16782-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16782-1-AP (THEM2 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using THEM2 Polyclonal antibody (16782-1-AP)

    IP experiment of HeLa using 16782-1-AP

    IP result of anti-THEM2 (IP:16782-1-AP, 4ug; Detection:16782-1-AP 1:2000) with HeLa cells lysate 1085 ug.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using THEM2 Polyclonal antibody (16782-1-AP)

    IHC staining of human lung cancer using 16782-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 16782-1-AP (THEM2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using THEM2 Polyclonal antibody (16782-1-AP)

    IHC staining of human lung cancer using 16782-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 16782-1-AP (THEM2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using THEM2 Polyclonal antibody (16782-1-AP)

    IF Staining of HepG2 using 16782-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using THEM2 antibody (16782-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).

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Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, mouse kidney tissue, mouse liver tissue
Positive IP detected inHeLa cells
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16782-1-AP targets THEM2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen THEM2 fusion protein Ag10212 相同性解析による交差性が予測される生物種
Full Name thioesterase superfamily member 2
Calculated molecular weight 140 aa, 15 kDa
Observed molecular weight 16 kDa
GenBank accession numberBC000894
Gene symbol THEM2
Gene ID (NCBI) 55856
RRIDAB_2287241
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Them2 (also named Acot13) is a homotetrameric fatty acyl-CoA thioesterase. Them2 is enriched in liver and oxidative tissues, including BAT, and is primarily associated with mitochondria.

Protocols

Product Specific Protocols
WB protocol for THEM2 antibody 16782-1-APDownload protocol
IHC protocol for THEM2 antibody 16782-1-APDownload protocol
IF protocol for THEM2 antibody 16782-1-APDownload protocol
IP protocol for THEM2 antibody 16782-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols