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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CTMP Polyclonal antibody

CTMP Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 14692-1-AP

Synonyms

THEM4, EC:3.1.2.2, Carboxyl-terminal modulator protein, Acyl-coenzyme A thioesterase THEM4, Acyl-CoA thioesterase THEM4

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse brain tissue using CTMP Polyclonal antibody (14692-1-AP)

    WB analysis of mouse brain using 14692-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 14692-1-AP (CTMP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using CTMP Polyclonal antibody (14692-1-AP)

    WB analysis of HepG2 using 14692-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 14692-1-AP (CTMP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse skeletal muscle tissue using CTMP Polyclonal antibody (14692-1-AP)

    WB analysis of mouse skeletal muscle using 14692-1-AP

    mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 14692-1-AP (CTMP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using CTMP Polyclonal antibody (14692-1-AP)

    IP experiment of mouse brain using 14692-1-AP

    IP result of anti-CTMP (IP:14692-1-AP, 4ug; Detection:14692-1-AP 1:500) with mouse brain tissue lysate 4000ug.

  • Immunohistochemistry (IHC) staining of human endometrial cancer tissue using CTMP Polyclonal antibody (14692-1-AP)

    IHC staining of human endometrial cancer using 14692-1-AP

    Immunohistochemical analysis of paraffin-embedded human endometrial cancer using 14692-1-AP (CTMP antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human endometrial cancer tissue using CTMP Polyclonal antibody (14692-1-AP)

    IHC staining of human endometrial cancer using 14692-1-AP

    Immunohistochemical analysis of paraffin-embedded human endometrial cancer using 14692-1-AP (CTMP antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CTMP Polyclonal antibody (14692-1-AP)

    IF Staining of HeLa using 14692-1-AP

    Immunofluorescent analysis of Hela cells, using CTMP antibody 14692-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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Tested Applications

Positive WB detected inmouse brain tissue, HepG2 cells, mouse skeletal muscle tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman endometrial cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHela cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14692-1-AP targets CTMP in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CTMP fusion protein Ag6244 相同性解析による交差性が予測される生物種
Full Name thioesterase superfamily member 4
Calculated molecular weight 27 kDa
Observed molecular weight 26 kDa
GenBank accession numberBC065277
Gene symbol CTMP
Gene ID (NCBI) 117145
RRIDAB_2271685
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for CTMP antibody 14692-1-APDownload protocol
IHC protocol for CTMP antibody 14692-1-APDownload protocol
IF protocol for CTMP antibody 14692-1-APDownload protocol
IP protocol for CTMP antibody 14692-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IP

Heliyon

PRAME promotes proliferation of multiple myeloma cells through CTMP/Akt/p21/CCND3 axis by ubiquitinating CTMP and p21

Authors - Kai Sun
View Article