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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using THOC3 Polyclonal antibody (11716-1-AP)

    WB analysis using 11716-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 11716-1-AP (THOC3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using THOC3 Polyclonal antibody (11716-1-AP)

    IHC staining of human ovary cancer using 11716-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 11716-1-AP (THOC3 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

THOC3 Polyclonal antibody

THOC3 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 11716-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

Tho3, TEX1, hTREX45

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHEK-293 cells
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11716-1-AP targets THOC3 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen THOC3 fusion protein Ag2289 相同性解析による交差性が予測される生物種
Full Name THO complex 3
Calculated molecular weight 327 aa, 36 kDa
Observed molecular weight39 kDa
GenBank accession numberBC006849
Gene Symbol THOC3
Gene ID (NCBI) 84321
RRIDAB_3085379
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96J01
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

THOC3 is a component of the nuclear THO transcription elongation complex, which is a part of the larger transcription export (TREX) complex that couples messenger RNA processing and export. Additionally, THOC3 plays a key role in many cellular mechanisms, such as in the pathogenesis of cancer and neurodegenerative diseases (PMID: 36674977). THOC3 has 2 isoforms with the molecular weights of 36 and 39 kDa.

Protocols

Product Specific Protocols
WB protocol for THOC3 antibody 11716-1-APDownload protocol
IHC protocol for THOC3 antibody 11716-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols